Goat anti Mouse IgG1 Alexa Fluor 488
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Catalog number43R-1649
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Price:
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Size500 µg
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CategorySecondary Antibody
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Antibody SubtypeConjugated Secondary Antibodies
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Area of researchImmunology
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Raised inGoat
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SpecificityMouse IgG1 Fc gamma specific
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Cross ReactivityMinimal cross reactivity with human, bovine and rabbit serum proteins. Does not react with other mouse IgG subtypes, IgM or the Fab portion of mouse IgG1.
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IsotypesIgG1
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Conjugate TagAlexa Fluor 488
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Method of PurificationGoat anti Mouse IgG1 was purified by affinity chromatography
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Concentration1.4 mg/ml
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Form BufferProvided as a lyophilized powder in 0.01M sodium phosphate, 0.25 M NaCl, pH 7.6, and 0.05% NaN3, with 15 mg/ml BSA.
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StorageStore at 2-8 deg C
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Shipping conditionsBlue Ice
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Tested forELISA; Immunoassays
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Usage RecommendationsDilution Range of 1:100 - 1:800 for most applications
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PropertiesFor facs or microscopy Alexa 1 conjugate. Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Goat anti Mouse IgG1 has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable.
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ConjugationAlexa Fluor
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
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Latin nameCapra aegagrus hircus, Mus musculus
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Gene target
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Short nameGoat anti Mouse IgG1
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Techniqueanti mouse, Mouse, anti, Goat, antibody to, anti mouse antibodies, goats, mouses
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HostGoat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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IsotypeIgG1
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LabelALEXA FLUOR 488
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SpeciesMouse, Goats, Mouses
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Alternative nameGoat anti Mouse IgG1 secondary antibody (Alexa Fluor 488)
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Alternative techniquemurine, antibodies, caprine
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data