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Goat anti Mouse IgG1 Alexa Fluor 488

  • Catalog number
    43R-1649
  • Product name
    Goat anti Mouse IgG1 Alexa Fluor 488
  • Size
    500 µg
  • Price
    537EUR
  • Supplier
    fitzgerald
  • Ask
  • Category
    Secondary Antibody
  • Antibody Subtype
    Conjugated Secondary Antibodies
  • Area of research
    Immunology
  • Raised in
    Goat
  • Specificity
    Mouse IgG1 Fc gamma specific
  • Cross Reactivity
    Minimal cross reactivity with human, bovine and rabbit serum proteins. Does not react with other mouse IgG subtypes, IgM or the Fab portion of mouse IgG1.
  • Isotypes
    IgG1
  • Conjugate/Tag
    Alexa Fluor 488
  • Method of Purification
    Goat anti Mouse IgG1 was purified by affinity chromatography
  • Concentration
    1.4 mg/ml
  • Form & Buffer
    Provided as a lyophilized powder in 0.01M sodium phosphate, 0.25 M NaCl, pH 7.6, and 0.05% NaN3, with 15 mg/ml BSA.
  • Storage
    Store at 2-8 deg C
  • Shipping conditions
    Blue Ice
  • Tested for
    ELISA; Immunoassays
  • Usage Recommendations
    Dilution Range of 1:100 - 1:800 for most applications
  • Properties
    For facs or microscopy Alexa 1 conjugate. Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Goat anti Mouse IgG1 has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable.
  • Conjugation
    Alexa Fluor
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
  • Latin name
    Capra aegagrus hircus, Mus musculus
  • Additional isotype
    IgG1
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Gene target
  • Short name
    Goat anti Mouse IgG1
  • Technique
    anti mouse, antibody to, anti mouse antibodies, goats, mouses
  • Host
    Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
  • Label
    ALEXA FLUOR 488
  • Species
    Mouse, Goats, Mouses
  • Alternative name
    caprine antibody to Mouse IgG1 Alexa Fluor 488
  • Alternative technique
    murine
  • Ask
MESH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    classification, economics, history, instrumentation, methods, standards, trends, utilization, veterinary, statistics & numerical data, ethics
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