Chicken anti Mouse IgG H + L biotin
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Catalog number43R-1300
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Price:
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Size500 µg
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CategorySecondary Antibody
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Antibody SubtypeConjugated Secondary Antibodies
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Area of researchImmunology
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Type of ImmunogenPurified Mouse IgG (H&L)
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Raised inChicken
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SpecificityThis antibody reacts with heavy (gamma) chains on mouse IgG and light chains on all mouse immunoglobulins.
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Cross ReactivityNo reactivity is observed to non-immunoglobulin mouse serum proteins or light chains on all human immunoglobulins or IgG from human or rabbit or serum proteins from human or rabbit.
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Conjugate TagBiotin
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Grade PurityGrater than 95% pure
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Method of PurificationAffinity purified using solid phase Mouse IgG (H&L)
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SourceIg fraction was prepared using eggs obtained from healthy hens of US origin.
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Concentration1.0 mg/ml (lot specific)
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Form BufferClear, colorless liquid, containing 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1% (w/v) BSA and 0.05% (w/v) NaN3. Protease/IgG free, pH 7.2.
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StorageStore at 2-8 deg C.
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Shipping conditionsBlue Ice
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Tested forPlease refer to the datasheet
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Usage Recommendations1:2000-1:20,000 ELISA/WB, 1:500-1:5000 IHC
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
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PropertiesBiotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Chickens like all bird species have IgY antibodies that are very stable and found in the yolk. Gallus Gallus domestica is the Latin name of the Chicken.
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ConjugationBiotinylated
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AboutImmunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target-
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Gene symbolCALD1, FUT1, L3MBTL2
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Short nameanti Mouse IgG H + L
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Techniqueanti mouse, Mouse, anti, IgG, antibody to, anti mouse antibodies, IgGs, mouses
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Hostmouse
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IsotypeIgG, IgG
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LabelBiotin
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SpeciesMouse, Chickens, Mouses
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Alternative nameChicken anti Mouse IgG (H + L) secondary antibody (biotin)
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Alternative techniquemurine, antibodies, immunoglobulins
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Gene info
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Identity
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Gene
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Long gene namecaldesmon 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1992-07-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namefucosyltransferase 1 (H blood group)
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Synonyms gene
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Synonyms gene name
- fucosyltransferase 1 (galactoside 2-alpha-L-fucosyltransferase, Bombay phenotype included)
- fucosyltransferase 1 (galactoside 2-alpha-L-fucosyltransferase)
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Synonyms name
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Locus
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Discovery year1989-06-06
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Entrez gene record
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RefSeq identity
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Classification
- Fucosyltransferases
- Blood group antigens
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameL3MBTL histone methyl-lysine binding protein 2
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Synonyms gene name
- l(3)mbt-like 2 (Drosophila)
- L3MBTL2, polycomb repressive complex 1 subunit
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Synonyms
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GenBank acession
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Locus
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Discovery year2002-04-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- MBT domain containing
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data