Annexin V-FITC Reagent
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Catalog number1001-1000
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PricePlease ask
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Size1000 assays
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DescriptionFITC conjugated Annexin V for detection of apoptosis through fluorescence microscopy or by flow cytometry. Reagent for detecting early stages of apoptosis. During apoptosis, phosphatidylserine (PS) is translocated from the cytoplasmic face of the plasma membrane to the cell surface. Annexin V has a strong, Ca²ᶧ-dependent affinity for PS and therefore is used as a probe for detecting apoptosis. The Annexin V-FITC conjugate can be used for detection of apoptosis by fluorescence microscopy or by flow cytometry.
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Product Highlights•Detection method- flow cytometry (Ex/Em = 488/530 nm) using FL1 channel and fluorescence microscopy •Sample- Cell culture •Formulation- 0.15 mg/ml in PBS containing 1% BSA, 0.02% sodium azide •Simple, easy to use and convenient
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Storage Temp+4°C
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Shippinggel pack
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Shelf Life12 months
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NotesFor research use only. Not to be used for human or animal treatment or consumption.
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Additional descriptionThis Annexin V calcium dependent phospholipid binding protein will be downstream of BCL2 pathway and at caspase apoptotic level. Biovision has more cell death kits and antibodies.
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PropertiesThis Biovision Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Biovision FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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Gene target
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Short nameAnnexin V-FITC Reagent
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TechniqueFITC, Fluorescein
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LabelFITC
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Alternative nameAnnexin V-fluorecein Reagent
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Alternative techniquefluorescine
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MeSH Data
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Name
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ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data