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Annexin V-FITC Reagent

  • Catalog number
  • Product name
    Annexin V-FITC Reagent
  • Size
    1000 assays
  • Price
    1 132.00 EUR
  • Supplier
  • Highlights
    •Detection method- flow cytometry (Ex/Em = 488/530 nm) using FL1 channel and fluorescence microscopy •Sample- Cell culture •Formulation- 0.15 mg/ml in PBS containing 1% BSA, 0.02% sodium azide •Simple, easy to use and convenient
  • Description
    Reagent for detecting early stages of apoptosis. During apoptosis, phosphatidylserine (PS) is translocated from the cytoplasmic face of the plasma membrane to the cell surface. Annexin V has a strong, Ca²ᶧ-dependent affinity for PS and therefore is used as a probe for detecting apoptosis. The Annexin V-FITC conjugate can be used for detection of apoptosis by fluorescence microscopy or by flow cytometry.
  • Category
    Annexin V Apoptosis Assay
  • Storage condition
  • Shipping condition
    gel pack
  • Maximum time for storage
    12 months
  • Additional description
    This Annexin V calcium dependent phospholipid binding protein will be downstream of BCL2 pathway and at caspase apoptotic level. Biovision has more cell death kits and antibodies.
  • Properties
    This Biovision Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Biovision FITC is highly sensitive to pH extremes.
  • Conjugation
    Anti-FITC Antibody
  • Gene target
  • Short name
    Annexin V-FITC Reagent
  • Technique
    FITC, Fluorescein
  • Label
  • Alternative name
    Annexin V-fluorecein Reagent
  • Alternative technique
  • Name
  • Concept
    Scope note:Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
  • Tree numbers
    • E01.370.225.500.607.512.240
    • E01.370.225.750.551.512.240
    • E05.200.500.607.512.240
    • E05.200.750.551.512.240
    • E05.478.583.375
  • Qualifiers
    classification, economics, history, instrumentation, methods, standards, trends, utilization, veterinary, statistics & numerical data, ethics
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