Recombinant Bovine GM-CSF

Tech file
Type

Yeast recombinant proteins with good glycosilation in smaal amounts for use as standard in ELISA kits.
Gene

Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts that functions as a cytokine. The pharmaceutical analogs of naturally occurring GM-CSF are called sargramostim and molgramostim. Reconstitution It is recommended that sterile ddH2O containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of not less than 1 mg/mL of the cytokine.
Source

Recombinants or rec. proteins
Group

recombinants

Gene target

GM-CSF
Gene symbol

CSF2, PPP1R3A, CSF1, CSF3, IL3
Short name

Recombinant GM-CSF
Technique

Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Immunochemistry kits advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
Species

Bovine, Bos Taurus genes are available form the Bovine Genome database.
Alternative name

Rec. Bovine GM-CSF
Alternative technique

rec

Identity

HGNC:9291
Gene

PPP1R3A
Long gene name

protein phosphatase 1 regulatory subunit 3A
Synonyms gene
- PPP1R3
Synonyms gene name
- protein phosphatase 1, regulatory (inhibitor) subunit 3A (glycogen and sarcoplasmic reticulum binding subunit, skeletal muscle)
- protein phosphatase 1, regulatory (inhibitor) subunit 3A
- protein phosphatase 1, regulatory subunit 3A
Synonyms
- GM
Synonyms name
- glycogen-associated regulatory subunit of protein phosphatase-1
- protein phosphatase 1 regulatory subunit GM
GenBank acession
- AF024578
Locus

7q31.1
Discovery year

1993-01-22
Entrez gene record

5506
Pubmed identfication
- 7926294
RefSeq identity
- NM_002711
Classification
- Protein phosphatase 1 regulatory subunits
VEGA ID

OTTHUMG00000156944

Identity

HGNC:2438
Gene

CSF3
Long gene name

colony stimulating factor 3
Synonyms gene
- GCSF
- G-CSF
- C17orf33
Synonyms gene name
- chromosome 17 open reading frame 33
- colony stimulating factor 3 (granulocyte)
Synonyms
- MGC45931
Synonyms name
Locus

17q21.1
Discovery year

2001-06-22
Entrez gene record

1440
Pubmed identfication
- 3499671
- 3501046
RefSeq identity
- NM_172220
Classification
- Receptor ligands
VEGA ID

OTTHUMG00000133247

Name

Primary Cell Culture
Concept

Scope note: The initial culturing of cells derived directly from fresh TISSUES.
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data