Iscove's Modified Dulbecco's Medium (IMDM) With L-Glutamine, 25mM HEPES Buffer and Sodium bicarbonate Without Glucose and Sodium bicarbonate
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Catalog numberAL230A-40X500ML
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Price:
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Size40X500ML
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DescriptionIscove's Modified Dulbecco's Medium (IMDM) With L-Glutamine, 25mM HEPES Buffer and Sodium bicarbonate Without Glucose and Sodium bicarbonate, is an orangish red colored, clear solution. When it is appropriately supplemented, it supports good growth of precursor cells of erythrocytes and macrophages. It also supports good growth of T and B lymphocytes and a variety of hybrid cells under serum free or reduced serum conditions.
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Shelf life12 months.
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StorageStore at 2-8°C away from bright light
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AppearanceOrangish red colored, clear solution
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PH7.00 -7.60
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Osmolality in mOsm Kg H2O280.00 -320.00
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SterilityNo bacterial or fungal growth is observed after 14 days of incubation, as per USP specification.
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Cultural ResponseThe growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts and comparing it with a control medium.
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Endotoxin ContentNMT 5EU/ml
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UsesLaboratory chemicals, Manufacture of substances
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Personal ProtectionUse personnel protective equipment. Wear disposable gloves, dust mask and eye protection. Avoid dust formation.
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Toxicological InformationNA
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OrderingTo order Iscove's Modified Dulbecco's Medium (IMDM) With L-Glutamine, 25mM HEPES Buffer and Sodium bicarbonate Without Glucose and Sodium bicarbonate, please use the Cat. Nr.AL230A-40X500ML and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
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TipsOur specialists recommend you to follow carefully the pre-registered instructions for Iscove's Modified Dulbecco's Medium (IMDM) With L-Glutamine, 25mM HEPES Buffer and Sodium bicarbonate Without Glucose and Sodium bicarbonate
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Additional descriptionBuffering solutions are useful to keep the pH range sable when using this reagent of Himedia. There are two major types of growth media those used for cell culture , which use specific cell types derived from plants or animals, and microbiological culture, which are used for growing microorganisms, such as bacteria or yeast. The most common growth media for microorganisms are nutrient broths and agar plates with antibiotics; specialized media are sometimes required for microorganism and cell culture growth.
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Buffer25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
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Groupmedia
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Gene target
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Short nameIscove's Modified Dulbecco's Medium (IMDM) With L-Glutamine, 25mM Sodium bicarbonate Without Glucose Sodium bicarbonate
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TechniqueMedium, cell culture Media
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Alternative nameIscove's Modified Dulbecco's Medium (IMDM) including L-Glutamine, 25mM HEPES solution and Sodium bicarbonate Without Glucose and Sodium bicarbonate
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Alternative techniquecellculture
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Virushhv
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MeSH Data
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Name
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ConceptScope note: Methods to study reactions or processes taking place in an artificial environment outside the living organism.
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Tree numbers
- E05.481
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data