HEV I & III Real Time RT-PCR Kit

Storage temperature

store cold
Expiry date

1 y
Category

research antibodies, ELISAs, recombinant proteins, vectors and reagents for laboratory use
Group

PCR, polymerase chain reaction, RT-PCR mixes
About

TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
Properties

Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.

Gene target

HEV III Real Time PCR
Gene symbol

CPLX3, MGAT3, MGST3, IL33, P4HA3, CASP5, THAP4, IGHVIII-82, MT-CSB3, IGHVIII-67-4
Short name

HEV I & III Real Time RT-PCR Kit
Technique

RT-PCR, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment.
Alternative name

HEV I & III Real Time Reverse transcription PCR test kit reagent
Alternative technique

rtpcrkits, kits, dna-amplification
Alternative to gene target

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Identity

HGNC:7046
Gene

MGAT3
Long gene name

beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase
Synonyms gene name
- mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase
Synonyms
- GNT-III
GenBank acession
- D13789
Locus

22q13.1
Discovery year

1993-08-27
Entrez gene record

4248
Pubmed identfication
- 8370666
RefSeq identity
- NM_002409
Classification
- Mannosyl-glycoprotein N-acetylglucosaminyltransferases
VEGA ID

OTTHUMG00000030185

Identity

HGNC:30135
Gene

P4HA3
Long gene name

prolyl 4-hydroxylase subunit alpha 3
Synonyms gene name
- procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide III
- prolyl 4-hydroxylase, alpha polypeptide III
Synonyms
- C-P4Halpha(III)
Synonyms name
- collagen prolyl 4-hydroxylase alpha(III)
GenBank acession
- AY327887
Locus

11q13.4
Discovery year

2004-05-12
Entrez gene record

283208
Pubmed identfication
- 14500733
RefSeq identity
- NM_182904
VEGA ID

OTTHUMG00000165254

Identity

HGNC:1506
Gene

CASP5
Long gene name

caspase 5
Synonyms gene name
- caspase 5, apoptosis-related cysteine protease
- caspase 5, apoptosis-related cysteine peptidase
Synonyms
- ICE(rel)III
Locus

11q22.3
Discovery year

1996-09-13
Entrez gene record

838
Pubmed identfication
- 7797592
- 9250871
RefSeq identity
- NM_004347
Classification
- Caspases
- Caspase recruitment domain containing
VEGA ID

OTTHUMG00000048073

Identity

HGNC:5709
Gene

IGHVIII-82
Long gene name

immunoglobulin heavy variable (III)-82 (pseudogene)
Synonyms gene name
- immunoglobulin heavy variable (III)-82
- immunoglobulin heavy variable (III)-82 pseudogene
Synonyms
- IGHV(III)-82
GenBank acession
- AB019437
Locus

14q32.33
Discovery year

2000-04-17
Entrez gene record

28338
RefSeq identity
- NG_001019
Classification
- Immunoglobulin heavy locus at 14q32.33
VEGA ID

OTTHUMG00000151732

Identity

HGNC:5707
Gene

IGHVIII-67-4
Long gene name

immunoglobulin heavy variable (III)-67-4 (pseudogene)
Synonyms gene name
- immunoglobulin heavy variable (III)-67-4
- immunoglobulin heavy variable (III)-67-4 pseudogene
Synonyms
- IGHV(III)-67-4
GenBank acession
- AB019437
Locus

14q32.33
Discovery year

2000-04-17
Entrez gene record

28340
RefSeq identity
- NG_001019
Classification
- Immunoglobulin heavy locus at 14q32.33
VEGA ID

OTTHUMG00000151874

Name

Polymerase Chain Reaction
Concept

Scope note: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Tree numbers
- E05.393.620.500
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data