GenCefe

GenCefe Biotech Co., Ltd. is a biotechnology company founded in 2020, with its headquarters located in the Wuxi Airport Economic Development Zone, Jiangsu Province, China. In 2021, the company expanded its operations by establishing a U.S. branch in California. GenCefe specializes in the development and application of molecular biology technologies, utilizing high-throughput automation and artificial intelligence to advance the gene synthesis industry.

Core Services and Technologies

GenCefe offers a comprehensive suite of services tailored for early-stage drug discovery, synthetic biology, and molecular diagnostics:

Gene Synthesis: Expertise in synthesizing complex, GC-rich, and long DNA sequences, with services including codon optimization, gene fragments, subcloning, and plasmid preparation.
RNA Synthesis: Production of various RNA molecules such as siRNA, miRNA, sgRNA, and circular RNA (circRNA), along with RNA modifications and quality control standards.
DNA Oligonucleotide Synthesis: Custom synthesis of DNA oligonucleotides with a range of modifications, including qPCR primers and probes, NGS primers, FISH probes, and PCR assay development.
Synthetic DNA Libraries: Creation of site-directed mutagenesis libraries, random mutant libraries, degenerate mutation libraries, and sgRNA libraries.
CRISPR-Cas9 Services: Synthesis of sgRNA, ssDNA, and sgRNA libraries to support gene editing applications.
Recombinant Protein Expression: Offering expression systems in bacterial, mammalian, insect (baculovirus), yeast, and cell-free platforms.
Peptide Synthesis and Modification: Custom peptide synthesis services, including modified, fluorescent-labeled, stable isotope-labeled, cyclic, and glycopeptides, as well as peptide libraries.
Reagents and Kits: Provision of various reagents and kits to support molecular biology research.