Area of research
Type of protein
Method of Purification
Streptavidin protein (Cy5) was purified by Protein A affinity chromatography.
Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% NaN3. I mMunoglobulin and protease free.
Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
ELISA; FC; IF
ELISA: 1:10,000-1:50,000, FC: 1:500-1:2,500, IF: 1:1,000-1:5,000
Cy5 antibodies are excited by the 650-nanometer wave of an argon laser and detected at a 670-nanometer captor.