Goat anti Mouse IgM (mu chain) (FITC)
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Catalog number43R-1370
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Price:
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Size2 mg
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ApplicationsFC, IF, IHC
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Concentration1.0 mg/ml
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Product SubtypeConjugated Secondary Antibodies
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Research AreaImmunology
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Product TypeSecondary Antibodies
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ImmunogenPurified Mouse IgM, u chain
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SpecificityThis antibody reacts with heavy (mu) chains on mouse IgM.
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Form BufferClear, fluorescent yellow liquid, containing 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1% (w/v) BSA and 0.05% (w/v) Sodium Azide. Protease/IgG free, pH 7.2.
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StorageStore at 2-8 deg C.
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Shipping InfoBlue Ice
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
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PropertiesThis fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes. Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
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ConjugationAnti-FITC Antibody
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Latin nameCapra aegagrus hircus, Mus musculus
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Gene target
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Gene symbolJCHAIN, AP2M1
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Short nameGoat anti Mouse IgM (mu chain) (FITC)
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Techniqueanti mouse, Mouse, anti, FITC, Goat, IgM, antibody to, anti mouse antibodies, Fluorescein, goats, IgMs, mouses
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HostGoat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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IsotypeNA, IgM
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LabelFITC
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SpeciesMouse, Goats, Mouses
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Alternative nameGoat anti Mouse IgM (mu chain) secondary antibody (FITC)
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Alternative techniquemurine, antibodies, caprine, fluorescine, immunoglobulin-m
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Gene info
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Identity
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Gene
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Long gene namejoining chain of multimeric IgA and IgM
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Synonyms gene
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Synonyms gene name
- immunoglobulin J polypeptide, linker protein for immunoglobulin alpha and mu polypeptides
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameadaptor related protein complex 2 subunit mu 1
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Synonyms gene
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Synonyms gene name
- adaptor related protein complex 2 mu 1 subunit
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2000-09-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Adaptor related protein complex 2
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data