Goat anti Mouse IgG H + L rhodamine
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Catalog number43R-1331
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Price:
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Size1 mg
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CategorySecondary Antibody
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Antibody SubtypeConjugated Secondary Antibodies
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Area of researchImmunology
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Type of ImmunogenPurified Mouse IgG (H&L)
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Raised inGoat
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SpecificityThis antibody reacts with heavy (gamma) chains on mouse IgG and light chains on all mouse immunoglobulins.
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Cross ReactivityNo reactivity is observed to non-immunoglobulin mouse serum proteins or serum proteins from bovine, horse, human, Pig, or rabbit.
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Conjugate TagRhodamine
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Grade PurityGrater than 95% pure
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Method of PurificationAffinity purified using solid phase Mouse IgG (H&L)
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SourceGoat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
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Concentration1.0 mg/ml (lot specific)
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Form BufferLyophilized from 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1% (w/v) BSA and 0.05% (w/v) NaN3. Protease/IgG free, pH 7.2. Dilute in dH2O to a concentration of 1 mg/ml
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StorageStore at 2-8 deg C.
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Shipping conditionsBlue Ice
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Tested forFC; IF; IHC
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Usage RecommendationsThe optimal working dilution should be determined by the end user. Suggested starting dilution(s): 1:20 – 1:2000 for most applications
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
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Latin nameCapra aegagrus hircus, Mus musculus
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AboutImmunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Gene target
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Short nameGoat anti Mouse IgG H + L rhodamine
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Techniqueanti mouse, Mouse, anti, Goat, IgG, antibody to, anti mouse antibodies, goats, IgGs, mouses
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HostGoat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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IsotypeIgG, IgG
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LabelRhodamine
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SpeciesMouse, Goats, Mouses
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Alternative nameGoat anti Mouse IgG (H + L) secondary antibody (rhodamine)
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Alternative techniquemurine, antibodies, caprine, immunoglobulins
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data