Goat anti Human Lambda Chain biotin
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Catalog number43C-CB9132
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Price:
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Size1 mg
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CategorySecondary Antibody
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Antibody SubtypeConjugated Secondary Antibodies
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Area of researchImmunology
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Type of ImmunogenGoat anti-human lambda chain (biotin) was raised in goat using human l lambda chain ..
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Raised inGoat
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SpecificityHuman lambda chain
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Cross ReactivityShould be determined by the end user
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Conjugate TagBiotin
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Method of PurificationGoat anti Human Lambda Chain (biotin) was purified by affinity chromatography.
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Form BufferLyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0 01% NaN3. I mMunoglobulin and protease free.
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StorageStore at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
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Shipping conditionsBlue Ice
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Tested forELISA; IHC; WB
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Usage RecommendationsELISA: 1:20,000-1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
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PropertiesBiotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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ConjugationBiotinylated
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Latin nameCapra aegagrus hircus
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Gene target
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Short nameGoat anti Lambda Chain
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Techniqueanti human, anti, Goat, antibody to, anti human antibodies, goats
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HostGoat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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LabelBiotin
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SpeciesHuman, Goats, Humans
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Alternative nameGoat anti Human Lambda Chain secondary antibody (biotin)
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Alternative techniqueantibodies, caprine
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data