Mouse IgG2a Isotype Control
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Catalog number10-458-C025
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Price:
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Size25ug
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Long nameMouse Monoclonal IgG2a Isotype Control
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AntigenMouse IgG2a Isotype Control
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Species reactivityImmunoglobulin Isotype Control
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Negative species reactivityHuman, Other species Not tested (expected negative), Porcine, Mouse, Rat
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ClonalityMonoclonal Antibody (Mab)
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ClonePPV-04
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ApplicationsFC, negative control
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ImmunogenIg Control
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Format conjugationazide free, unconjugated
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SpecificityThis mouse IgG2a monoclonal antibody (clone PPV-04) reacts with undefined epitope on a plant pathogen.
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Research areaImmunoglobulin Isotype Controls
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Concentration1 mg/ml
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Storage bufferAzide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 μm filter sterilized.
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Shipping and storageMouse Monoclonal IgG2a Isotype Control is shipped on blue ice or ice packs. Upon receipt store at 4 degrees Celcius. Do not freeze!
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BackgroundThe specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μg/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody PPV-04, generated against a plant pathogen, does not cross-react with other species, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG2a molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
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Purity> 95% (by SDS-PAGE)
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Purification methodPurified by protein-A affinity chromatography
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ReferencesContact us for the most recent list of references
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DescriptionIsotype or positive controls by peptides, antibodies and deactivated samples.
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PropertiesImmunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
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Groupisotypes
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Short nameIgG2a Isotype Control
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TechniqueControl, Isotype, Mouse, controls, Isotypes and controls, mouses
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HostMouse (Mus musculus)
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IsotypeIgG2a, IgG2a
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SpeciesMouse, Mouses
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Alternative nameMurine IgG2a Isotype Control
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Alternative techniquecontrol, controls, murine
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Tissuecontrol
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