T24 [T-24] Cell Line

#

• Catalog number
  EP-CL-0227
• Price:

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• Size
  1×10⁶ cells/vial

# #

• Abbreviation
  T24 [T-24]
• Leadtime
  3-5 days
• Shipping condition
  Dry ice
• Storage
  Liquid nitrogen -196°C
• Age
  NA
• Morphology
  Epithelial
• Growth_properties
  Adherent
• Doubling_time
  NA
• Background
  NA
• Biosafety
  1
• Medium
  McCoy's 5A (PM150710)+10% FBS (164210-500)+1% P/S (PB180120)
• Subculturing
  Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
• Ratio
  1:2-1:4
• Renewal
  Every 2 to 3 days
• Cryopreservation
  Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
• Culture_conditions
  Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
• Tumorigenic
  NA
• Effects
  NA
• Receptor_expression
  NA
• Antigen_expression
  NA
• Gene_expression
  NA
• Applications
  NA
• Duration
  NA
• Technical data
• Description
  For cells, cell lines and tissues in culture till half confluency.

#

• Gene target
  T24   [T-24]   Line  
• Gene symbol
  SNORD115-24, SNORD116-24, IGLV3-24, SNORD114-24, TBXT, PLS3, CD164, IGHV1-24, ERVW-24, IGKV2-24
• Short name
  T24 [T-24] Line
• Species
  Homo sapiens, Human
• Alternative name
  T24 [T-24] cellular Line
• Tissue
  Urinary bladder

Gene info

• Identity
  HGNC:33043
• Gene
  SNORD115-24
• Long gene name
  small nucleolar RNA, C/D box 115-24
• Synonyms
  • HBII-52-24
• Locus
  15q11.2
• Discovery year
  2010-10-05
• Entrez gene record
  100036563
• Pubmed identfication
  • 11106375
  • 11726556
• RefSeq identity
  • NR_003495
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:33090
• Gene
  SNORD116-24
• Long gene name
  small nucleolar RNA, C/D box 116-24
• Synonyms
  • HBII-85-24
• GenBank acession
  • AF250841
• Locus
  15q11.2
• Discovery year
  2007-02-15
• Entrez gene record
  100033435
• Pubmed identfication
  • 11106375
  • 11726556
• RefSeq identity
  • NR_003338
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:5907
• Gene
  IGLV3-24
• Long gene name
  immunoglobulin lambda variable 3-24 (pseudogene)
• Synonyms gene name
  • immunoglobulin lambda variable 3-24
  • immunoglobulin lambda variable 3-24 pseudogene
• GenBank acession
  • X71968
• Locus
  22q11.22
• Discovery year
  2000-05-08
• Entrez gene record
  28794
• RefSeq identity
  • NG_000002
• Classification
  • Immunoglobulin lambda locus at 22q11.2
• VEGA ID
  OTTHUMG00000151230

Gene info

• Identity
  HGNC:33012
• Gene
  SNORD114-24
• Long gene name
  small nucleolar RNA, C/D box 114-24
• Synonyms
  • 14q(II-24)
• Locus
  14q32.31
• Discovery year
  2006-09-26
• Entrez gene record
  767604
• Pubmed identfication
  • 12045206
• RefSeq identity
  • NR_003217.1
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:11515
• Gene
  TBXT
• Long gene name
  T-box transcription factor T
• Synonyms gene
  • T
• Synonyms gene name
  • T, brachyury homolog (mouse)
  • T brachyury transcription factor
• GenBank acession
  • AJ001699
• Locus
  6q27
• Discovery year
  1996-04-12
• Entrez gene record
  6862
• Pubmed identfication
  • 8963900
• RefSeq identity
  • NM_003181
• Classification
  • T-box transcription factors
• VEGA ID
  OTTHUMG00000015991

Gene info

• Identity
  HGNC:9091
• Gene
  PLS3
• Long gene name
  plastin 3
• Synonyms gene name
  • plastin 3 (T isoform)
• Synonyms
  • T-plastin
• GenBank acession
  • L05491
• Locus
  Xq23
• Discovery year
  1997-08-18
• Entrez gene record
  5358
• Pubmed identfication
  • 8428952
• Classification
  • EF-hand domain containing
• VEGA ID
  OTTHUMG00000022237
• Locus Specific Databases
  • Global Variome shared LOVD

Gene info

• Identity
  HGNC:1632
• Gene
  CD164
• Long gene name
  CD164 molecule
• Synonyms gene name
  • CD164 antigen, sialomucin
  • CD164 molecule, sialomucin
• Synonyms
  • MUC-24
  • MGC-24
  • DFNA66
• Synonyms name
  • deafness, autosomal dominant 66
• GenBank acession
  • AF106518
• Locus
  6q21
• Discovery year
  2000-06-08
• Entrez gene record
  8763
• Pubmed identfication
  • 9680353
  • 9763543
  • 26197441
• RefSeq identity
  • NM_006016
• Classification
  • CD molecules
• VEGA ID
  OTTHUMG00000015339
• Locus Specific Databases
  • LRG_1419

Gene info

• Identity
  HGNC:5551
• Gene
  IGHV1-24
• Long gene name
  immunoglobulin heavy variable 1-24
• GenBank acession
  • M99642
• Locus
  14q32.33
• Discovery year
  2000-04-04
• Entrez gene record
  28467
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000152095

Gene info

• Identity
  HGNC:49312
• Gene
  ERVW-24
• Long gene name
  endogenous retrovirus group W member 24
• Synonyms gene name
  • endogenous retrovirus group W, member 24
• GenBank acession
  • HG421038
• Locus
  12q13.13
• Discovery year
  2013-10-21

Gene info

• Identity
  HGNC:5781
• Gene
  IGKV2-24
• Long gene name
  immunoglobulin kappa variable 2-24
• GenBank acession
  • X12684
• Locus
  2p11.2
• Discovery year
  2000-04-18
• Entrez gene record
  28923
• RefSeq identity
  • NG_000834
• Classification
  • Immunoglobulin kappa locus at 2p11.2
• VEGA ID
  OTTHUMG00000151655

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