SW 780 [SW-780, SW780] Cell Line
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Catalog numberEP-CL-0449
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PricePlease ask
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Size1×10⁶ cells/vial
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AbbreviationSW 780 [SW-780, SW780]
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Leadtime3-5 days
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Shipping conditionDry ice
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StorageLiquid nitrogen -196°C
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Age80 years
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MorphologyEpithelial
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Growth_propertiesAdherent
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Doubling_timeNA
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BackgroundThe patient had preoperative chemotherapy(Thiotepa).The cells have a reported plating efficiency of 41%.
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Biosafety1
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MediumDMEM (PM150210)+10% FBS (164210-500)+1% P/S (PB180120)
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SubculturingRemove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
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Ratio1:2-1:4
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RenewalEvery 2 to 3 days
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CryopreservationFreeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
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Culture_conditionsAtmosphere: Air, 95%; CO2, 5% Temperature: 37℃
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TumorigenicYes
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EffectsYes, forms tumors in nude mice.
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Receptor_expressionNA
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Antigen_expressionNA
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Gene_expressionNA
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ApplicationsNA
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DurationNA
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DescriptionFor cells, cell lines and tissues in culture till half confluency.
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Gene target
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Gene symbolTUBB, MEX3D, SLC4A1, CDK5RAP3
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Short nameSW 780 [SW-780, SW780] Line
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SpeciesHomo sapiens, Human
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Alternative nameSW 780 [SW-780, SW780] cellular Line
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TissueUrinary bladder
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Gene info
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Identity
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Gene
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Long gene nametubulin beta class I
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Synonyms gene name
- tubulin, beta polypeptide
- tubulin, beta
- tubulin, beta class I
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2004-11-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Tubulins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemex-3 RNA binding family member D
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Synonyms gene
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Synonyms gene name
- ring finger and KH domain containing 1
- mex-3 homolog D (C. elegans)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2004-03-17
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ring finger proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesolute carrier family 4 member 1 (Diego blood group)
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Synonyms gene
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Synonyms gene name
- Waldner blood group
- erythrocyte membrane protein band 3
- Diego blood group
- solute carrier family 4, anion exchanger, member 1 (erythrocyte membrane protein band 3, Diego blood group)
- solute carrier family 4 (anion exchanger), member 1
- solute carrier family 4 (anion exchanger), member 1 (Diego blood group)
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Synonyms
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Synonyms name
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Locus
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Discovery year1988-04-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Blood group antigens
- Solute carriers
- CD molecules
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameCDK5 regulatory subunit associated protein 3
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2002-07-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
