MC3T3-E1 Subclone 14 Cell Line
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Catalog numberEP-CL-0378
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PricePlease ask
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Size1×10⁶ cells/vial
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AbbreviationMC3T3-E1 Subclone 14
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Leadtime3-5 days
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Shipping conditionDry ice
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StorageLiquid nitrogen -196°C
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AgeNewborn
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MorphologyFibroblast
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Growth_propertiesAdherent
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Doubling_timeNA
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BackgroundThe subclones were selected for high or low osteoblast differentiation and mineralization after growth in medium containing ascorbic acid. The MC3T3-E1 Subclone 4 and the MC3T3 Subclone 14 lines exhibit high levels of osteoblast differentiation after growth in ascorbic acid and 3 to 4mM inorganic phosphate.
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Biosafety1
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MediumMEMα (PM150421)+10% FBS (164210-500)+1% P/S (PB180120)
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SubculturingRemove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
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Ratio1:2-1:4
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RenewalEvery 2 to 3 days
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CryopreservationFreeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
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Culture_conditionsAtmosphere: Air, 95%; CO2, 5% Temperature: 37℃
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TumorigenicYes
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EffectsYes, in immunodeficient mice.
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Receptor_expressionNA
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Antigen_expressionNA
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Gene_expressionCollagen
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ApplicationsThese cell lines are good models for studying in vitro osteoblast differentiation, particularly ECM signaling. They have behavior similar to primary calvarial osteoblasts.
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DurationNA
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DescriptionFor cells, cell lines and tissues in culture till half confluency.
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Gene target
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Gene symbolRNU105C, SNORA73A, RNU105B, ENOPH1, BCHE, MAGED4, ERVE-1, GATA1, OGDH, PCBP1
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Short nameMC3T3-E1 Subclone 14 Line
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SpeciesMus musculus, Mouse
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Alternative nameMC3T3-E1 Subclone 14 cellular Line
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Clone name14
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TissueBone/calvaria
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Gene info
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Identity
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Gene
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Long gene nameRNA, U105C small nucleolar
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-12-14
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Entrez gene record
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RefSeq identity
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, H/ACA box 73A
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Synonyms gene
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Synonyms gene name
- RNA, U17a small nucleolar
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Synonyms
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GenBank acession
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Locus
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Discovery year1993-08-24
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, H/ACA box
Gene info
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Identity
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Gene
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Long gene nameRNA, U105B small nucleolar
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-12-14
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Entrez gene record
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RefSeq identity
Gene info
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Identity
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Gene
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Long gene nameenolase-phosphatase 1
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Synonyms
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Synonyms name
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Locus
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Discovery year2007-01-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- HAD Asp-based non-protein phosphatases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namebutyrylcholinesterase
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Synonyms gene
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Synonyms gene name
- cholinesterase 1
- cholinesterase (serum) 2
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Synonyms
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameMAGE family member D4
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Synonyms gene name
- melanoma antigen family D4
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-12-23
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- MAGE family
- Small nucleolar RNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameendogenous retrovirus group E member 1
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Synonyms gene
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Synonyms gene name
- endogenous retroviral sequence E, 1
- endogenous retrovirus group E, member 1
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-06-04
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Entrez gene record
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Pubmed identfication
Gene info
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Identity
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Gene
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Long gene nameGATA binding protein 1
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Synonyms gene
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Synonyms gene name
- GATA-binding protein 1 (globin transcription factor 1)
- globin transcription factor 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1990-09-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- GATA zinc finger domain containing
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameoxoglutarate dehydrogenase
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Synonyms gene name
- oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)
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Synonyms
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GenBank acession
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Locus
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Discovery year1989-06-30
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Entrez gene record
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Pubmed identfication
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Classification
- Oxoglutarate dehydrogenase family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namepoly(rC) binding protein 1
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Synonyms gene name
- poly(rC)-binding protein 1
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Synonyms
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Synonyms name
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Locus
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Discovery year1996-06-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Heterogeneous nuclear ribonucleoproteins
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VEGA ID
