B16-F10 Cell Line

Abbreviation

B16-F10
Leadtime

3-5 days
Shipping condition

Dry ice
Storage

Liquid nitrogen -196°C
Age

NA
Morphology

Mixture of spindle-shaped and epithelial-like cells
Growth_properties

Adherent
Doubling_time

NA
Background

NA
Biosafety

1
Medium

DMEM (PM150210)+10% FBS (164210-500)+1% P/S (PB180120)
Subculturing

Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio

1:2-1:4
Renewal

Every 2 to 3 days
Cryopreservation

Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture_conditions

Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Tumorigenic

NA
Effects

NA
Receptor_expression

NA
Antigen_expression

NA
Gene_expression

NA
Applications

Transfection host.
Duration

NA
Technical data
Description

For cells, cell lines and tissues in culture till half confluency.