A-431 Cell Line

Abbreviation

A-431
Leadtime

3-5 days
Shipping condition

Dry ice
Storage

Liquid nitrogen -196°C
Age

85 years
Morphology

Epithelial
Growth_properties

Adherent
Doubling_time

~80-100 hours
Background

The A-431 cell line was established by D.J. Giard in 1973.
Biosafety

1
Medium

DMEM (PM150210)+10% FBS (164210-500)+1% P/S (PB180120)
Subculturing

Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio

1:3-1:6
Renewal

Every 2 to 3 days
Cryopreservation

Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture_conditions

Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Tumorigenic

Yes
Effects

Yes, forms rapidly growing subcutaneous tumors in immunosuppressed mice and colonies in soft agar.
Receptor_expression

NA
Antigen_expression

NA
Gene_expression

NA
Applications

Transfection host.
Duration

NA
Technical data
Description

For cells, cell lines and tissues in culture till half confluency.

Gene target

A-431 Line
Gene symbol

MIR431, HLA-A, IGHV5-10-1, CIDEA, RPA1, CDC37P1, OR1Q1, FABP4, LINC01139
Short name

A-431 Line
Species

Homo sapiens, Human
Alternative name

A-431 cellular Line
Tissue

Skin, epidermis

Identity

HGNC:5661
Gene

IGHV5-10-1
Long gene name

immunoglobulin heavy variable 5-10-1
Synonyms gene
- IGHV5-A
Synonyms gene name
- immunoglobulin heavy variable 5-A (provisional, gene/pseudogene)
- immunoglobulin heavy variable 5-A (provisional)
Synonyms
- IGHV5-a
GenBank acession
- X92227
Locus

14q32.33
Discovery year

2000-04-04
Entrez gene record

28386
RefSeq identity
- NG_001019
Classification
- Immunoglobulin heavy locus at 14q32.33
VEGA ID

OTTHUMG00000191143

Identity

HGNC:10289
Gene

RPA1
Long gene name

replication protein A1
Synonyms gene name
- replication protein A1 (70kD)
- replication protein A1, 70kDa
Synonyms
- REPA1
- RPA70
- HSSB
- RF-A
- RP-A
GenBank acession
- M63488
Locus

17p13.3
Discovery year

1993-12-14
Entrez gene record

6117
Pubmed identfication
- 8454588
RefSeq identity
- NM_002945
Classification
- Nucleotide excision repair
VEGA ID

OTTHUMG00000090579

Identity

HGNC:34406
Gene

CDC37P1
Long gene name

cell division cycle 37 pseudogene 1
Synonyms gene name
- cell division cycle 37 homolog (S. cerevisiae) pseudogene 1
Synonyms
- A-761H5.2
Locus

16p11.2
Discovery year

2009-01-29
Entrez gene record

390688
Pubmed identfication
- 15616553
RefSeq identity
- NG_009463
VEGA ID

OTTHUMG00000175739