5637 Cell Line

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• Catalog number
  EP-CL-0002
• Price:

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• Size
  1×10⁶ cells/vial

# #

• Abbreviation
  5637
• Leadtime
  3-5 days
• Shipping condition
  Dry ice
• Storage
  Liquid nitrogen -196°C
• Age
  68 years adult
• Morphology
  Epithelial
• Growth_properties
  Adherent
• Doubling_time
  ~24 hours
• Background
  The 5637 cell line has been established from the primary bladder carcinoma(grade Ⅱ) ofa patient by Dr. G. Cannon in 1974.
• Biosafety
  1
• Medium
  RPMI-1640 (PM150110)+10% FBS (164210-500)+1% P/S (PB180120)
• Subculturing
  Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
• Ratio
  1:3-1:6
• Renewal
  2 to 3 times per week
• Cryopreservation
  Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
• Culture_conditions
  Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
• Tumorigenic
  Yes
• Effects
  Yes, within 21 days at 100% frequency(5/5) in nude mice inoculated subcutaneously with 10^7 cells.
• Receptor_expression
  NA
• Antigen_expression
  NA
• Gene_expression
  NA
• Applications
  Transfection host.
• Duration
  NA
• Technical data
• Description
  For cells, cell lines and tissues in culture till half confluency.

#

• Gene target
  5637   Line  
• Short name
  5637 Line
• Species
  Homo sapiens, Human
• Alternative name
  5637 cellular Line
• Tissue
  Urinary bladder

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