FITC-Dextran, 4 kDa, 25 mg/ml x 5 ml

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• Catalog number
  4009
• Price:

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• Size
  1 vial

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• Tech file
• Data sheet
• References
  ELISA kits for collagen and chondrocytes more
• Other chondrocytes and synoviocytes
• References of collagen and chondrocyte research and assays
  Kim W.­U., Yoo W.­H., Won Park, Kang Y.­M., Kim S.­I., Park J.­H., Lee S.­S., Joo Y.­S, Min J.­  K., Hong Y.­S., Lee S.­H., Park S.­H., Cho C.­S., Kim H.­Y. IgG antibodies to type II collagen reflect inflammatory activity in patients with rheumatoid arthritis. Journal of Rheumatology, 2000; 27(3):575­581. Terato K., DeArmey D.A., Ye X.J., Griffiths M.M., Cremer M.A. The Mechanism of Autoantibody  Formation to Cartilage in Rheumatoid Arthritis: Possible Cross­Reaction of Antibodies to Dietary  Collagens with Autologous Type II Collagen. Clin Imm Immunopath, 1996; 79(2):142­154(13)  Terato K, Shimozuru Y, Katayama K, et al. Specificity of antibodies to type II collagen in rheumatoid arthritis. Arthritis Rheum 1990;33:1493­500. Clague RB, Moore LJ. IgG and IgM antibody to native type II collagen in rheumatoid serum and synovial fluid: evidence for the presence of collagen­anticollagen immune complexes in synovial fluid. Arthritis Rheum 1984;27:1370­7. Fujii K, Tsuji M, Kitamura A, Murota K. The diagnostic significance of anti­type II collagen antibody assay in rheumatoid arthritis. Int Orthop 1992;16:272­6.
• Properties
  This Chondrocytes and collagens Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Chondrocytes and collagens FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• Description
  The kilo Daltons subunit weight of FITC-Dextran, 4 , 25 mg/ x 5 compared to your protein ladder can be shifted a little due to electrophoresis effects. 1 kDa = 1000 g/mol protein

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• Gene target
  Dextran  
• Short name
  FITC-Dextran, 4 , 25 mg/ x 5
• Technique
  FITC, Fluorescein
• Label
  FITC
• Alternative name
  fluorecein-Dextran, 4 kiloDalton, 25 mg/milliliter x 5 milliliter
• Alternative technique
  fluorescine

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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