AAV-2 Helper Free Bicistronic Expression System (Puro)

• Catalog number
  VPK-415-SER2
• Price
  2228.24 USD
• Size
  1 test kit

• We send this reagant on
  Blue Ice
• The temperatureerature for storage is
  -20ºC
• Technical file
• SDS or safty data sheet
  more (VPK-410 through VPK-430-SER6) GHS.pdf
• Type of reagent
  Viral Expression
• Related techniques
  Viral Expression, Adeno-Associated Virus (AAV) Expression, AAV Helper Free Complete Expression Systems, AAV-2 Helper Free Complete Expression Systems
• Use of the reagent
  The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).
• Cellular use
  Complete Expression System contains packaging plasmids, expression vector and GFP control vector to produce recombinant AAV serotype 2
• Protocol information
  pHelper plasmid contains required E2A, E4, and VA RNA adenoviral genes; eliminates the need for a helper adenovirus
• Reagent properties
  Cloning capacity of expression vector = 1.8 kb
• Living modified organisme in this reagent or LMO
  Not applicable
• Species compatible to
  Humans
• Use of this test kit or product
  Genetics lab
• Antigen
  a specific protein sequence
• Buffer
  The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,17
• Molecular weight
  in kilo Danlto or kDa
• This reagent is purified
  0.97

• Gene target
  AAV-2   Helper   Free   Bicistronic   Expression   Puro  
• Gene symbol
  AAVS1, MIR1289-2, MIR521-2, MIR509-2, MIR512-2, MIR7-2, MIR329-2, RNU6-2, KRTAP13-2, SNORD114-2
• Short name
  AAV-2 Helper Free Bicistronic Expression System (Puro)
• Label
  puro
• Alternative name
  AAV-2 Helper Free Bicistronic Expression System (Puro)

Gene info

• Identity
  HGNC:22
• Gene
  AAVS1
• Long gene name
  adeno-associated virus integration site 1
• Synonyms
  • AAV
• GenBank acession
  • S51329
• Locus
  19q13
• Discovery year
  1990-09-10
• Entrez gene record
  17
• Pubmed identfication
  • 1334463

Gene info

• Identity
  HGNC:35282
• Gene
  MIR1289-2
• Long gene name
  microRNA 1289-2
• Synonyms gene
  • MIRN1289-2
• Synonyms
  • hsa-mir-1289-2
• Locus
  5q31.1
• Discovery year
  2008-11-03
• Entrez gene record
  100302134
• RefSeq identity
  • NR_031621
• Classification
  • MicroRNA MIR1289 family

Gene info

• Identity
  HGNC:32113
• Gene
  MIR521-2
• Long gene name
  microRNA 521-2
• Synonyms gene
  • MIRN521-2
• Synonyms
  • hsa-mir-521-2
• Locus
  19q13.42
• Discovery year
  2005-07-15
• Entrez gene record
  574481
• RefSeq identity
  • NR_030203
• Classification
  • MicroRNA MIR515 family

Gene info

• Identity
  HGNC:33641
• Gene
  MIR509-2
• Long gene name
  microRNA 509-2
• Synonyms gene
  • MIRN509-2
• Synonyms
  • hsa-mir-509-2
• Locus
  Xq27.3
• Discovery year
  2007-10-24
• Entrez gene record
  100126301
• RefSeq identity
  • NR_030586
• Classification
  • MicroRNA MIR506 family

Gene info

• Identity
  HGNC:32091
• Gene
  MIR512-2
• Long gene name
  microRNA 512-2
• Synonyms gene
  • MIRN512-2
• Synonyms
  • hsa-mir-512-2
• Locus
  19q13.42
• Discovery year
  2005-07-15
• Entrez gene record
  574459
• RefSeq identity
  • NR_030181
• Classification
  • MicroRNA MIR506 family

Gene info

• Identity
  HGNC:31639
• Gene
  MIR7-2
• Long gene name
  microRNA 7-2
• Synonyms gene
  • MIRN7-2
• Synonyms
  • hsa-mir-7-2
• Locus
  15q26.1
• Discovery year
  2004-04-23
• Entrez gene record
  407044
• RefSeq identity
  • NR_029606
• Classification
  • MicroRNA MIR7 family

Gene info

• Identity
  HGNC:32051
• Gene
  MIR329-2
• Long gene name
  microRNA 329-2
• Synonyms gene
  • MIRN329-2
• Synonyms
  • hsa-mir-329-2
• Locus
  14q32.31
• Discovery year
  2005-07-11
• Entrez gene record
  574409
• RefSeq identity
  • NR_029968
• Classification
  • MicroRNA MIR329 family

Gene info

• Identity
  HGNC:34270
• Gene
  RNU6-2
• Long gene name
  RNA, U6 small nuclear 2
• Synonyms
  • U6-2
• Locus
  19p13.3
• Discovery year
  2008-06-10
• Entrez gene record
  103625684
• Pubmed identfication
  • 12711679
• RefSeq identity
  • NR_125730
• Classification
  • Small nuclear RNAs

Gene info

• Identity
  HGNC:18923
• Gene
  KRTAP13-2
• Long gene name
  keratin associated protein 13-2
• Synonyms
  • KAP13-2
• GenBank acession
  • AP001708
• Locus
  21q22.11
• Discovery year
  2003-02-21
• Entrez gene record
  337959
• Pubmed identfication
  • 12359730
• Classification
  • Keratin associated proteins
• VEGA ID
  OTTHUMG00000057793

Gene info

• Identity
  HGNC:32990
• Gene
  SNORD114-2
• Long gene name
  small nucleolar RNA, C/D box 114-2
• Synonyms
  • 14q(II-2)
• Locus
  14q32.31
• Discovery year
  2006-09-26
• Entrez gene record
  767578
• Pubmed identfication
  • 12045206
• RefSeq identity
  • NR_003194.1
• Classification
  • Small nucleolar RNAs, C/D box

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