Routine DNA amplification
Amplify defined DNA regions for screening, verification, cloning or downstream analysis.
Molecular Biology
PCR Reagents
PCR reagents are essential components for DNA amplification workflows in molecular biology. They support routine PCR, high-fidelity amplification, endpoint PCR, qPCR preparation, cloning, genotyping, pathogen research, sequencing preparation and assay development. A successful PCR reaction depends on enzyme performance, buffer chemistry, magnesium concentration, primer design, template quality and thermal cycling conditions. Gentaur helps laboratories compare PCR reagents for workflows that require clean amplification, reproducible results and reliable reaction setup.
Product catalog
Browse PCR Reagents
Showing 25 unique product names from this subcategory.
2x PCR Blue Mix
SKU: TBS4005|Size: 2.0 mL
Aspergillus Flavus Taqman PCR Detection, 100 reactions
SKU: TBS42021-100|Size: 100 Tests
Aspergillus Fumigatus Taqman PCR Detection, 100 reactions
SKU: TBS42022-100|Size: 100 Tests
One-Step Sybr qRT-PCR, No ROX
SKU: TBS4007NR|Size: 100 Reactions
Aspergillus Terreus Taqman PCR Detection, 100 reactions
SKU: TBS42024-100|Size: 100 Tests
TriboTM 2x Genotyping PCR Ready Mix
SKU: TBS4003|Size: 2.5 mL
Presto™ PCR Cleanup 96 Well Binding Plate
SKU: 96DBP005|Size: each
2X All-In-One High Specificity PCR MasterMix Solution, 50 µLx96 RXNS
SKU: ABP-PP-MM02961|Size: 96 RXNS
10x PCR Buffer with MgCl2
SKU: ABP-PP-ERBFM05|Size: 5x1ml
Taq PCR Master Mix (2X, Red Dye)
SKU: BS9298|Size: 5ml
2X Pfu PCR Premix (5 x 1mL)
SKU: 9K-002-0022|Size: 5X1mL, 5ml
2X High-Taq PCR Premix (5 x 1mL)
SKU: 9K-002-0011|Size: 5X1mL, 5ml
8 Strip 0.2ml PCR Tube (Without Cap), 120/Bag
SKU: BP546-N|Size: 1PK, 120UNIT
Green-2-Go 1-Step qRT-PCR Mastermix-Rox (100X20ul Rxn)
SKU: QPCR010-R|Size: 100Rxn
96-Well PCR Tube Racks, Blue
SKU: R541-B|Size: 1 UNIT
PCR Clean Up for DNA Sequencing
SKU: BT5100|Size: 100preps
Gel and PCR Extraction System (100 preps)
SKU: 9K-006-0007|Size: 100preps
MyTaq Blood PCR Mix, 2x
SKU: BIO-25053/S|Size: Sample
Block, 40 x 0.2ml tubes or 5 PCR strips of 8 tubes each
SKU: BSH100-02|Size: 1 Each
Block, 96 x 0.2ml or one PCR plate
SKU: H5000-02|Size: 1 Each
MyBlock ll™- digital dry bath with 2 Quick-Flip blocks (BSWCMB) for tubes (0.2 to 2.0ml, PCR strips and PCR plates, 115V
SKU: BSH5002-2B|Size: 1 Each
StripSpin™ 12 Mini Centrifuge, holds 12 position PCR strips, 230V
SKU: C1248-E|Size: 1 PC
Tube Adapter, hold 96 x 0.2ml or 8 and 12 position PCR strips, 2/pk.
SKU: C2000-A02|Size: 1 Each
StripSpin™12D Digital Mini Centrifuge, holds 12 position PCR strips, 100-240V (European 2 prong plug)
SKU: C2248-E|Size: 1 Each
Tube holder, 96 x 0.2ml, 12 x PCR strips or one PCR plate (R2024 and H2024 Only)
SKU: R2020-PCR|Size: 1 Each
Overview
Core reagents for reliable DNA amplification
PCR reagents usually include DNA polymerases, reaction buffers, dNTPs, magnesium components, additives, master mixes, primers or workflow-specific amplification components. The correct combination affects yield, specificity, sensitivity and error rate.
Routine PCR may prioritize robust amplification and ease of use, while cloning, mutagenesis or sequencing preparation may require high-fidelity enzymes with lower error rates.
Researchers should match PCR reagent choice to template complexity, amplicon length, GC content, downstream application and required accuracy.
Applications
Common PCR reagent applications
High-fidelity PCR
Use proofreading polymerases when sequence accuracy is important for cloning or mutagenesis workflows.
Genotyping and screening
Support genetic analysis, colony screening, insertion checks and sample comparison workflows.
Pathogen research workflows
Amplify target sequences in research workflows involving microbial or viral nucleic acid detection.
Sequencing preparation
Prepare amplified DNA fragments for Sanger sequencing, library workflows or verification steps.
Selection guide
How to choose PCR reagents
Select PCR reagents according to polymerase type, template quality, amplicon length, fidelity requirements and downstream use.
1
Choose the polymerase type
Use standard polymerases for routine PCR and proofreading polymerases when high sequence accuracy is required.
2
Check amplicon length
Long targets may require enzymes and buffers optimized for long-range amplification.
3
Review template complexity
GC-rich templates, degraded DNA or low-copy targets may require specialized buffers or additives.
4
Compare master mix formats
Ready-to-use master mixes reduce pipetting steps and can improve consistency across reactions.
5
Match downstream workflow
Choose reagents compatible with cloning, sequencing, qPCR, gel analysis or diagnostic research workflows.
6
Control contamination risk
Use clean setup practices, controls and dedicated reagents for contamination-sensitive PCR workflows.
FAQ
PCR Reagents FAQ
Common questions about selecting PCR reagents for molecular biology workflows.
What are PCR reagents used for?
PCR reagents are used to amplify specific DNA sequences for research workflows such as cloning, genotyping, screening, sequencing preparation and molecular analysis.
What is included in PCR reagents?
PCR workflows commonly use DNA polymerase, buffer, dNTPs, magnesium, primers, template DNA and sometimes additives or master mixes.
When should I use high-fidelity PCR reagents?
Use high-fidelity reagents when amplified DNA will be cloned, sequenced, mutated or used in workflows where sequence accuracy matters.
Why does template quality matter in PCR?
Poor-quality or contaminated DNA can reduce amplification efficiency, increase background or cause failed reactions.
How can I reduce PCR contamination?
Use separate setup areas, filtered tips, negative controls, clean reagents and careful handling to reduce contamination risk.
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