Secondary antibody detection
Use goat anti-rabbit, goat anti-mouse or goat anti-human antibodies for signal detection.
Antibody Host Species
Goat antibodies are commonly used as both primary and secondary immunoreagents in protein detection workflows. Goat polyclonal antibodies are especially important for secondary antibody production because they can recognize immunoglobulins from species such as human, rabbit, mouse, rat and others. These antibodies support western blotting, immunohistochemistry, immunocytochemistry, immunofluorescence, ELISA and flow cytometry. Conjugated goat antibodies are available in formats such as HRP, biotin, FITC, cyanine dyes and Alexa Fluor dyes for flexible detection strategies.

Product catalog
Showing 25 unique product names from this subcategory.

AffiniPure Goat Anti-Mouse IgG H&L (min X Hu, Bov, Hrs, Rb, Sw Sr Prot), IRDye 800CW conjugated
SKU: GTR18624141|Size: 100 µL

Goat anti-Human IgA Coated - 96 well plates - 8 Well Strips on 12 x 8 frame - Clear PS
SKU: MT43F4-AHA|Size: 10x 96 Well Plates

Goat anti-Human IgA Coated - 96 well plates - 8 Well Breakable Strips on single well holding frame - Clear PS
SKU: MG43F-AHA|Size: 10x 96 Well Plates

Goat anti-Human IgG Coated - 96 well plates - 8 Well Strips on 12 x 8 frame - White PS
SKU: MTW40F4-AHG|Size: 10x 96 Well Plates

CF®498 Goat Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed, single label for STORM, 1 mg/mL
SKU: #20997-50uL|Size: 1x 50 µL

CF®583R Goat Anti-Mouse IgG (H+L), Highly Cross-Adsorbed (min X Rat), 2 mg/mL
SKU: #20903-50uL|Size: 1x 50 µL

CF®405S Goat Anti-Mouse IgG (H+L), Highly Cross-Adsorbed (min X Rat), 2 mg/mL
SKU: #20830-50uL|Size: 1x 50 µL

CF®535ST Goat Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed, single label for STORM, 1 mg/mL
SKU: #20822-500uL|Size: 1x 500 µL

CF®660C Goat Anti-Mouse IgG (H+L), Highly Cross-Adsorbed, single label for STORM, 1 mg/mL
SKU: #20812-50uL|Size: 1x 50 µL

CF®647 Goat Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed, single label for STORM, 1 mg/mL
SKU: #20809-500uL|Size: 1x 500 µL

CF®568 Goat Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed, single label for STORM, 1 mg/mL
SKU: #20801-50uL|Size: 1x 50 µL

CF®568 Goat Anti-Mouse IgG (H+L), Highly Cross-Adsorbed, single label for STORM, 1 mg/mL
SKU: #20800-50uL|Size: 1x 50 µL

CF®583R Goat Anti-Mouse IgG (H+L), Highly Cross-Adsorbed, single label for STORM, 1 mg/mL
SKU: #20792-50uL|Size: 1x 50 µL

CF®660C Goat Anti-Mouse IgG (H+L), Highly Cross-Adsorbed (min X Rat), 2 mg/mL
SKU: #20368-500uL|Size: 1x 500 µL

CF®633 Goat Anti-Mouse IgG (H+L), Highly Cross-Adsorbed (min X Rat), 2 mg/mL
SKU: #20341-50uL|Size: 1x 50 µL

CF®594 Goat Anti-Mouse IgG (H+L), Highly Cross-Adsorbed (min X Rat), 2 mg/mL
SKU: #20303-1|Size: 1x 50 µL

Goat anti-Rat IgG (H&L) Coated Polystyrene Particles, Cross-adsorbed, 5% w/v, 6.0-8.0 µm, 5mL
SKU: RTPXA5-60-5|Size: 5 mL

UltraPolymer Goat anti-Rabbit IgG (H&L) Conjugated to HRP, affinity purified, min x w/human, mouse or rat serum
SKU: MBS689581-01|Size: 15 mL

UltraPolymer Goat anti-Mouse IgG (H&L) Conjugated to HRP, affinity purified, min x w/bovine, horse, human, pig or rabbit serum proteins
SKU: MBS689578-01|Size: 15 mL

Goat anti-Mouse IgG (H&L) - Affinity Pure, DyLight633 Conjugate, min x w/bovine, horse, human, pig or rabbit serum proteins
SKU: MBS688188-01|Size: 1 mg

Donkey anti-Mouse IgG (H&L) - F(ab)'2 fragment, HRP Conjugate, min x w/bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep IgG
SKU: MBS688140-01|Size: 0.5 mg

Goat anti-Human IgA (alpha chain) - Affinity Pure, DyLight550 Conjugate, min x w/bovine, mouse, rabbit serum
SKU: MBS688119-01|Size: 1 mg

Goat anti-Rabbit IgG (H&L) - Affinity Pure, DyLight650 Conjugate, min x w/bovine, human, mouse IgG or serum proteins
SKU: MBS688050-01|Size: 1 mg

Donkey anti-Mouse IgG (H&L) - F(ab)'2 fragment, ALP Conjugate, min x w/bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep IgG
SKU: MBS688047-01|Size: 0.5 mg

Goat anti-Human IgG Fc, F(ab)'2 Fragment (min x w/ Human IgA/IgM, Bovine, Horse, Mouse, Rabbit Serum)
SKU: MBS689528-01|Size: 0.5 mg
Overview
Goat-derived secondary antibodies are widely used because they provide strong signal amplification and broad compatibility with common primary antibody host species.
Cross-adsorbed goat secondary antibodies help reduce unwanted binding to related species, which is important in multiplex panels and tissue staining workflows.
When choosing goat antibodies, researchers should check target species, immunoglobulin specificity, conjugate type, cross-adsorption profile and assay validation.
Applications
Use goat anti-rabbit, goat anti-mouse or goat anti-human antibodies for signal detection.
Support chemiluminescent, chromogenic or fluorescent blot detection workflows.
Use fluorescent goat secondary antibodies for microscopy and multiplex staining.
Support cell marker detection with compatible fluorophore-conjugated reagents.
Selection guide
Select goat antibodies according to target species, antibody class, conjugate, cross-adsorption and detection platform.
Choose goat anti-rabbit, anti-mouse, anti-human, anti-rat or another specificity according to your primary antibody.
Decide whether you need heavy and light chain recognition, Fc-specific detection or subclass-specific recognition.
Select HRP, biotin, FITC, Alexa Fluor, cyanine dye or alkaline phosphatase formats according to the assay.
Cross-adsorbed goat antibodies help reduce non-specific species reactivity in multiplex or multi-species assays.
Confirm suitability for western blot, ELISA, IHC, ICC, IF or flow cytometry.
FAQ
Common questions about goat host antibodies and secondary detection.
Goat hosts produce strong polyclonal responses against immunoglobulins from many species, making them useful for secondary detection.
It means the antibody was raised in goat and recognizes rabbit immunoglobulins, often used to detect rabbit primary antibodies.
Cross-adsorbed antibodies are processed to reduce unwanted binding to other species, improving specificity in complex panels.
Choose HRP for chemiluminescent or chromogenic detection, and fluorescent dyes for imaging or flow cytometry workflows.
Related categories