Gene knockout studies
Disrupt target genes to study loss-of-function effects and identify biological roles in cells or model systems.
CRISPR
CRISPR Gene Editing Tools
CRISPR gene editing tools support targeted genome modification, gene regulation and functional genomics research. These tools include Cas9-related reagents, CRISPR/Cas plasmids, guide RNA systems, detection antibodies and workflow components used to design, deliver, validate and analyze gene editing experiments. Researchers use CRISPR tools to investigate gene function, create knockout models, modulate gene expression, study regulatory regions and screen biological pathways. A successful CRISPR workflow depends on guide design, delivery method, Cas system, cell type, controls and validation strategy.
Product catalog
Browse CRISPR Gene Editing Tools
Showing 25 unique product names from this subcategory.
Olfr1489 CRISPR/Cas9 KO Plasmid (m)
SKU: sc-434756|Size: 20 µg
ALX1 CRISPR/Cas9 KO Plasmid (h)
SKU: B2015379|Size: 10 µg
INTS1 CRISPR/Cas9 KO Plasmid (h)
SKU: sc-409878|Size: 20 µg
PAK4 CRISPR/Cas9 KO Plasmid (m)
SKU: sc-427750|Size: 20 µg
SCOCO CRISPR/Cas9 KO Plasmid (m)
SKU: sc-425115|Size: 20 µg
DOC4 CRISPR/Cas9 KO Plasmid (h)
SKU: sc-406806|Size: 20 µg
PHF19 CRISPR/Cas9 KO Plasmid (h)
SKU: sc-408215|Size: 20 µg
TP53TG3 CRISPR/Cas9 KO Plasmid (h)
SKU: sc-418579|Size: 20 µg
Vav2 CRISPR/Cas9 KO Plasmid (h2)
SKU: sc-401490-KO-2|Size: 20 µg
lymphotactin CRISPR/Cas9 KO Plasmid (m)
SKU: sc-421461|Size: 20 µg
Vmn2r91 CRISPR/Cas9 KO Plasmid (m)
SKU: sc-437073|Size: 20 µg
DnaJB5 CRISPR/Cas9 KO Plasmid (h)
SKU: sc-406047|Size: 20 µg
MYL9 CRISPR/Cas9 KO Plasmid (h)
SKU: sc-401577|Size: 20 µg
CLDND2 CRISPR/Cas9 KO Plasmid (m)
SKU: sc-428820|Size: 20 µg
Gem CRISPR/Cas9 KO Plasmid (m)
SKU: sc-420533|Size: 20 µg
FOXI3 CRISPR/Cas9 KO Plasmid (m)
SKU: sc-433134|Size: 20 µg
TMEM8C CRISPR/Cas9 KO Plasmid (h)
SKU: sc-402487|Size: 20 µg
HoxA4 CRISPR/Cas9 KO Plasmid (h)
SKU: sc-405914|Size: 20 µg
Olfr609 CRISPR/Cas9 KO Plasmid (m)
SKU: sc-435154|Size: 20 µg
ABCG1 CRISPR/Cas9 KO Plasmid (m2)
SKU: sc-418933-KO-2|Size: 20 µg
Nup205 CRISPR/Cas9 KO Plasmid (h)
SKU: sc-404728|Size: 20 µg
G6pd2 CRISPR/Cas9 KO Plasmid (m)
SKU: sc-420445|Size: 20 µg
SARA CRISPR/Cas9 KO Plasmid (m)
SKU: sc-432954|Size: 20 µg
LRRC66 CRISPR/Cas9 KO Plasmid (m)
SKU: sc-433055|Size: 20 µg
FIBCD1 CRISPR/Cas9 KO Plasmid (h)
SKU: sc-413736|Size: 20 µg
Overview
Targeted tools for genome editing and gene regulation
CRISPR gene editing workflows use sequence-guided targeting to direct Cas enzymes or modified Cas systems to specific nucleic acid regions. Depending on the design, researchers can disrupt genes, introduce targeted changes, activate transcription, repress expression or study regulatory elements.
Gene editing experiments require more than one reagent. A complete workflow may include guide RNA design, Cas expression or delivery, plasmids or ribonucleoprotein systems, selection markers, validation primers, antibodies and downstream assays to confirm editing efficiency and biological effect.
Choosing the right CRISPR tool depends on the experimental goal. Knockout studies, CRISPR activation, CRISPR interference, reporter models and screening experiments all require different design choices, controls and validation methods.
Applications
Common CRISPR research applications
Functional genomics
Connect genes to phenotypes by testing how targeted genetic changes affect pathways, cell behavior or assay readouts.
Gene regulation
Use modified CRISPR systems to activate, repress or modulate transcription without necessarily cutting DNA.
CRISPR validation
Confirm Cas expression, guide delivery, target editing or downstream protein changes using molecular and immunodetection methods.
Screening workflows
Support pooled or targeted guide-based screens for pathway discovery, drug response and target validation.
Selection guide
How to choose CRISPR gene editing tools
Select CRISPR tools according to the editing goal, cell system, delivery method, Cas format, guide design and validation plan.
1
Define the editing goal
Clarify whether the experiment requires knockout, knock-in, activation, repression, base editing, screening or target validation.
2
Choose the Cas system
Select Cas9 or another Cas format based on target sequence, PAM requirements, delivery strategy and editing objective.
3
Plan guide RNA design
Evaluate guide specificity, target position, predicted off-target risk and compatibility with the selected Cas system.
4
Match the delivery method
Choose plasmid, viral, RNA or RNP delivery depending on cell type, expression duration, editing efficiency and workflow constraints.
5
Use proper controls
Include non-targeting guides, positive controls, mock-treated samples and rescue or validation controls where appropriate.
6
Validate editing results
Confirm editing by PCR, sequencing, qPCR, western blot, immunostaining, phenotype analysis or functional assays.
7
Check downstream compatibility
Make sure the selected CRISPR tools are compatible with your cell model, selection system, reporter assay or screening workflow.
FAQ
CRISPR Gene Editing Tools FAQ
Common questions about selecting CRISPR reagents for genome editing and functional genomics research.
What are CRISPR gene editing tools used for?
CRISPR gene editing tools are used to target specific nucleic acid sequences for gene knockout, gene regulation, functional genomics, screening and model generation research.
What is the role of Cas9 in CRISPR workflows?
Cas9 is a programmable nuclease guided by RNA to a target DNA sequence. It can introduce targeted breaks or be modified for non-cutting regulatory applications.
How do I choose a guide RNA?
Guide RNA selection should consider target sequence, PAM compatibility, predicted specificity, target region, off-target risk and the biological goal of the experiment.
What controls are important in CRISPR experiments?
Important controls include non-targeting guides, positive-control guides, untreated or mock-treated samples and validation controls for editing or expression changes.
How can CRISPR editing be validated?
CRISPR editing can be validated using PCR, Sanger sequencing, next-generation sequencing, qPCR, western blotting, immunostaining or functional phenotype assays.
Related categories