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Mouse TIMP-1 PicoKine ELISA Kit

  • Catalog number
    EK0521
  • Product name
    Mouse TIMP-1 PicoKine ELISA Kit
  • Size
    1x96 well plate
  • ELISA s application
    For quantitative measurement of mouse TIMP-1 in cell culture supernatant, cell lysate, tissue homogenates, serum and plasma heparin, EDTA .
  • Gene tested
    murine TIMP-1
  • ELISA standard curve
    Concentration(pg/ml) + 0 + 31.2 + 62.5 + 125 + 250 + 500 + 1000 + 2000 O.D. + 0.004 + 0.097 + 0.186 + 0.374 + 0.638 + 1.117 + 1.734 + 2.522
  • ELISA kit detection limits
    31.2pg/ml to 2000pg/ml
  • ELISA s sensitivity
    < 10pg/ml
  • Target of this ELISA
    Natural and recombinant mouse TIMP-1
  • Cross reaction
    the ELISA does not cross react with any other proteins except from closely related species
  • ELISA s precision parameters
    intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);371;602;1025;382;619;1270 ELISA standard deviations 15.95;29.5;36.9;24.45;47.04;73.66 CV(%);4.3;4.9;3.6;6.4;7.6;5.8
  • ELISA kit description
    GENTAUR's mouse TIMP-1 ELISA test is a ready to use ELISA kit. We use a monoclonal from rat specific for TIMP-1 was used as capure antibodies on the ELISA test plate. The internal standards (NSO, C25-R205) and your samples are measured with a biotin conjugated polyclonal from goat specific for TIMP-1 will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more mouse TIMP-1 in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
  • Detector and capure antibodies
    A rat monoclonal detector antibodies is used with a goat biotin polyconal capture antibodies.
  • The ELISA contains
    Catalog number;Description;Quantity -;96-well plate precoated with anti- mouse TIMP-1 antibodies;1 ST0000-10;Lyophilized recombinant mouse TIMP-1 standard;10ng/tube×2 AR1107;Biotinylated anti- mouse TIMP-1 antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex(ABC);130ul(dilutions 1:100) AR1106-1;Sample diluent buffers;30ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
  • Anti coagulation
    heparin or EDTA
  • How the elisa works
    Sample the amount of 100ul/well of the standard solution at 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml mouse TIMP-1 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernatant, serum or plasma(heparin, EDTA) to the ELISA plate. Keep in mind that each mouse TIMP-1 standard solution and each sample be measured in duplicate.
  • Minutes of TMB incubation
    20-25
  • More gene info
    TIMP metallopeptidase inhibitor 1, also known as TIMP1, a tissue inhibitor of metalloproteinases, is a glycoprotein that is expressed from the several tissues of organisms. This protein is a member of the TIMP family. It was found to reside at Xp11.4-p11.1. This is the first growth factor found to be X-linked. The glycoprotein is a natural inhibitor of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. What’s more, TIMP1 is thought to play a regulatory role in connective tissues by forming inactive complexes with those metalloproteinases that are normally responsible for connective tissue turnover.
  • Antigen
    NSO, C25-R205
  • Keep at
    Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
  • Gene symbol
    TIMP1
  • Uniprot
    Metalloproteinase inhibitor 1
  • Gene name
    TIMP metallopeptidase inhibitor 1
  • Alternative
    C-C chemokine receptor type 5;C-C CKR-5;CC-CKR-5;CCR-5;CCR5;CCR5_HUMAN;CD195;CHEMR13;CLGI;Collagenase inhibitor;EPA;EPO;Erythroid potentiating activity;Fibroblast collagenase inhibitor;FLJ90373;HCI;HIV-1 fusion coreceptor;Human Collagenase Inhibitor;Metalloproteinase inhibitor 1;Metalloproteinase inhibitor 1 precursor;OTTHUMP00000023214;TIMP;TIMP 1;TIMP metallopeptidase inhibitor 1;TIMP1 protein;Tissue Inhibitor of metalloproteinase 1;Tissue inhibitor of metalloproteinases;Tissue inhibitor of metalloproteinases 1;Tissue inhibitor of metalloproteinase 1 erythroid potentiating activity collagenase inhibitor
  • Gen protein identity
    P12032
  • Entrez nr
    21857
  • Alternative to gene target
    • [ "v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog", "C-Kit and CD117 and PBT and SCFR", "KIT and IDBG-18980 and ENSG00000157404 and 3815", "transferase activity", "Extracellular", "Kit and IDBG-172083 and ENSMUSG00000005672 and 16590", "KIT and IDBG-642326 and ENSBTAG00000002699 and 280832" ]
  • Gene target
    TIMP-1 PicoKine
    • Gene info
    Locus Specific Databases:Global Variome shared LOVD
  • Host
    mouse, antigen assay ELISA kit 1 Kit (96 Wells)
  • Short name
    Mouse TIMP-1 PicoKine ELISA Kit
  • technique filter
    • ELISA kit
    • ELISA
    • Mouse
  • Technique
    ELISA kit, ELISA, Mouse, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests. mouses
  • Alternative technique
    murine, elisas, kits