Single-cell sequencing refers to a suite of molecular biology techniques designed to profile the genome, transcriptome, epigenome, or proteome of individual cells. This is typically achieved through:

1. Single-cell RNA sequencing (scRNA-seq): Captures messenger RNA (mRNA) transcripts in each cell.
2. Single-cell ATAC-seq: Profiles chromatin accessibility to assess epigenetic landscapes.
3. Single-cell multiomics: Simultaneously quantifies RNA and epigenomic states.
4. Single-cell immune profiling: Links T-cell or B-cell receptor sequences with gene expression.

Together, these methods elucidate how distinct cells function, communicate, and differentiate under physiological or pathological conditions.

The standard single-cell sequencing pipeline consists of the following stages:

1. Sample Preparation and Nuclei Isolation

Obtaining a high-quality cell or nuclei suspension is critical. Gentaur offers several optimized kits to isolate viable nuclei or remove dead cells while preserving molecular integrity:

1. Lysis-Based Nuclei Isolation Kit
2. Column-Based Nuclei Isolation Kit
3. Subcellular Fractionation Kit
4. Magnetic Bead-Based Dead Cell Removal Kit

These tools ensure compatibility with downstream gene expression and chromatin accessibility assays while maintaining the integrity of nuclear content.

2. Barcoding and Library Preparation

After isolation, single cells are encapsulated (e.g., in droplets), lysed, and barcoded using oligonucleotide primers. Each cell receives a unique molecular identifier (UMI), allowing for later demultiplexing. Gentaur supports this stage with trusted library construction reagents:

Gene Expression:

1. Single Cell 3’ v3.1 Kit
2. Gene Expression Flex Kit
3. Fixed RNA Profiling Kit
4. Dual Index Kit TT Set A

These kits are compatible with fixed or fresh samples and enable scalable gene expression profiling with high fidelity and reproducibility.

ATAC & Multiome:

1. Single Cell ATAC v2 Kit
2. Multiome ATAC + Gene Expression Kit
3. Barcoded ATAC Transposase
4. Dual Index Kit TS Set B

For researchers exploring chromatin states or combining RNA and ATAC readouts, these kits facilitate robust, high-throughput multiomic profiling.

Immune Profiling:

1. 5’ v2 Reagent Kit
2. V(D)J Enrichment Kit (Human B cells)
3. V(D)J Enrichment Kit (Human T cells)
4. Feature Barcode Kit for Cell Surface Protein

These immune profiling kits are essential for mapping antigen receptor diversity and surface protein expression at single-cell resolution, particularly in tumor microenvironments and autoimmune disorders.

Immunology

Understanding immune responses at the single-cell level is vital in cancer immunotherapy, vaccine development, and infectious disease research. Gentaur’s V(D)J enrichment kits and feature barcode kits empower scientists to decode clonality and T/B cell identity across thousands of individual cells.

Cancer Biology

Tumor tissues are composed of diverse cell types—malignant, immune, stromal, and vascular. By using Single Cell 3’ v3.1 or Multiome kits, researchers can dissect tumor heterogeneity, track therapeutic resistance, and identify cancer stem-like cells.

Neuroscience

The brain contains vast numbers of neurons and glial subtypes. With nuclei isolation kits and ATAC-seq tools, researchers can perform single-nucleus RNA-seq or ATAC-seq on frozen brain tissues to uncover epigenetic programs underlying cognition or neurodegeneration.

Stem Cell and Developmental Biology

Embryonic development involves intricate gene expression cascades. Gentaur’s Gene Expression Flex Kit supports time-resolved studies that reconstruct developmental trajectories and fate decisions using pseudotemporal ordering algorithms.

Infectious Disease

Single-cell technologies enable tracking of host-pathogen interactions, profiling of infected versus bystander cells, and mapping of antiviral gene programs—key in COVID-19, HIV, or tuberculosis research. Fixed RNA profiling from Gentaur is especially useful in high-biosafety contexts.

Single-cell datasets are sparse, high-dimensional, and noisy. Bioinformatic pipelines typically include:

1. Quality control: Filtering cells with low transcript counts.
2. Normalization: Correcting for technical variability.
3. Clustering and dimensionality reduction: Using UMAP or t-SNE.
4. Differential expression: Identifying marker genes.
5. Trajectory inference: Reconstructing cell lineage dynamics.

Many tools like Seurat, Scanpy, and Monocle are used in tandem with platforms like Cell Ranger or STARsolo. Gentaur's kits produce data that align with these analysis tools, ensuring seamless integration into bioinformatics pipelines.

With over 22 years of biotech sourcing expertise, Gentaur is trusted by scientists across academia and industry. Their kits are selected for performance, compatibility, and scalability—critical in large consortia and translational research projects. Whether it's immune profiling, transcriptional atlases, or nuclei preparation, Gentaur delivers high-quality reagents and global support.

As a Platinum Sponsor of SCSO 2025, Gentaur continues to support innovation in spatial biology, transcriptomics, and single-cell science.

Researchers registering through gentaur.com/register receive a 2.5% discount on the full range of products.

📍 Voortstraat 49 – Kampenhout 1910, Belgium

📞 +32 2 265 09 20

🌐 www.gentaur.com

📩 [email protected]

Single-cell sequencing is more than a method—it's a framework for understanding life at its most granular resolution. It challenges assumptions made from bulk tissue studies and opens new doors in precision science. With validated tools from Gentaur for nuclei isolation, gene expression, immune profiling, and chromatin analysis, researchers can perform rigorous, reproducible single-cell workflows that reveal previously hidden layers of biology.

Whether you are studying immunity, development, disease, or cellular identity, Gentaur’s products offer a reliable and scalable foundation for your single-cell experiments. As the field continues to grow, so does the need for partners that understand the scientific and technical demands of this transformative technology.