TIGAR-TAT, human recombinant
Catalog number
7218-50
Supplier
Price
655.66 USD
Size
50 ug
Synonyms
TP53-induced glycolysis and apoptosis regulator (TIGAR)
Alternative_names
TP53-induced glycolysis and apoptosis regulator (TIGAR)
Description
Catalyzes the hydrolysis of fructose-2-6 bisphosphate
Recombinant
Yes
Source
E. Coli
Purity by SDS PAGE
≥95%
Assay
SDS-PAGE
Endotoxin Level
< 0.1 ng/ug of protein (
Molecular Weight
31.6 kDa
Storage Temp
-20°C
Shipping
Gel Pack
Shelf Life
12 months
Appearance
Lyophilized powder
Physical form description
Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 7.5.
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Background Information
TIGAR is a p53-inducible enzyme that catalyzes the hydrolysis of fructose-2-6 bisphosphate (F-2-6-BP) to fructose-6-phosphate and inorganic phosphate. F-2-6-BP is a powerful activator of 6-phosphofructose-1 kinase, the rate limiting enzyme of glycolysis. By lowering the intracellular level of F-2-6-BP, TIGAR expression leads to increased glucose processing via the pentose phosphate pathway, the major cellular source for NADPH. Protein transduction using TAT fusion proteins represents an alternative methodology for introducing transcription factors and other intracellular proteins into primary as well as transformed cells. Recombinant human TIGAR-TAT expressed in E. coli is a 31.6 kDa protein containing 283 amino-acid residues, including the 269 residues of full-length TIGAR fused to a 14-residue C-terminal peptide containing the TAT transduction domain (GGGYGRKKRRQRRR).
Amino acid sequence
ARFALTVVRH GETRFNKEKI IQGQGVDEPL SETGFKQAAA AGIFLNNVKF THAFSSDLMR TKQTMHGILE RSKFCKDMTV KYDSRLRERK YGVVEGKALS ELRAMAKAAR EECPVFTPPG GETLDQVKMR GIDFFEFLCQ LILKEADQKE QFSQGSPSNC LETSLAEIFP LGKNHSSKVN SDSGIPGLAA SVLVVSHGAY MRSLFDYFLT DLKCSLPATL SRSELMSVTP NTGMSLFIIN FEEGREVKPT VQCICMNLQD HLNGLTETRG GGYGRKKRRQ RRR
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants
Gene target
Gene symbol
TAT, TIGAR, TAT-AS1, HTATSF1, HTATSF1P1, TRI-TAT2-2, TRI-TAT1-1, TRI-TAT3-1, TRI-TAT2-3, TRI-TAT2-1
Short name
TIGAR-TAT, recombinant
Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
Species
Human, Humans
Alternative name
TIGAR-TAT, H. sapiens Rec.
Alternative technique
rec
Gene info
Gene info
Gene info
Identity
Gene
Long gene name
TAT antisense RNA 1
GenBank acession
Locus
Discovery year
2014-10-16
Entrez gene record
RefSeq identity
Classification
VEGA ID
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
MeSH Data
Name
Concept
Scope note:The initial culturing of cells derived directly from fresh TISSUES.
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Similar products
