- Catalog numberP1116-500
- Product nameMutant Alanine Racemase Y354N, Active, Recombinant
- Size500 ug
- PriceAsk For Price
- SynonymsAlanine Racemase Y354N, alr, dal
- Alternative namesAlanine Racemase Y354N, alr, dal
- DescriptionCatalyzes the interconversion of L-alanine and D-alanine. Also weakly active on serine.
- RecombinantYes
- SourceE. Coli
- Purity by SDS PAGE> 99%
- AssaySDS-PAGE
- Activity Specifications test methodMeasured by its ability to convert 1 µmole of L-serine into D-serine per minute at 37 ºC and pH 7.4 in the presence of pyridoxal phosphate
- Biological activitySpecific activity is ≥ 50 mU/mg in which one unit enzyme converts 1 µmole of L-serine into D-serine per minute at 37 ºC and pH 7.4 in the presence of pyridoxal phosphate
- Results≥ 50 mU/mg
- Unit DefinitionOne Unit enzyme converts 1 µmole of L-serine into D-serine per minute at 37 ºC and pH 7.4 in the presence of pyridoxal phosphate
- Molecular Weight43 kDa
- Storage Temp-20°C
- ShippingGel pack
- Shelf Life12 months
- AppearanceLyophilized
- Physical form descriptionLyophilized form proprietary buffer
- Reconstitution InstructionsReconstitute enzyme in 50 mM phosphate buffer, pH 7.4.
- Background InformationMutant Alanine Racemase (Y354N) (mAR-Y354N), a pyridoxal 5-phosphate (PLP) dependent enzyme catalyzes the interconversion of the L-Serine to D-Serine. In WT Alanine Racemase Tyr354 plays a crucial role in defining the strict specificity of AR for alanine, in converting L-Alanine to D-Alanine, which is an important component of the peptidoglycan layer of bacterial cell wall. By mutating the active site Tyr 354 to Asn, the specificity of the enzyme changes and it becomes a racemase with dual specificity for L- Alanine and L-Serine.
- HandlingCentrifuge the vial prior to opening.
- UsageFor Research Use Only! Not to be used in humans
- Datasheet
- Gene targetMutant Alanine Racemase Y354N Active
- Short nameMutant Alanine Racemase Y354N, Active, Recombinant
- technique filter
- Recombinant
- TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
- Alternative techniquerec