Synonyms
FCGR2B, CD32b, CD32, FCG2, IGFR2, CDw32
Alternative_names
FCGR2B, CD32b, CD32, FCG2, IGFR2, CDw32
Description
A low affinity receptor for IgG
Recombinant
Yes
Source
HEK293 cells
Purity by SDS PAGE
≥95%
Assay
SDS-PAGE
Biological activity
Measured by its ability to bind human IgG with an estimated Kd
Results
Measured by its ability to bind human IgG with an estimated Kd
Molecular Weight
This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 20.2 kDa. The predicted N-terminus is Ala 46. DTT-reduced Protein migrates as 23-33 kDa due to different glycosylation.
Storage Temp
-20°C
Shipping
Gel Pack
Shelf Life
12 months
Appearance
Lyophilized
Physical form description
Lyophilized from 0.22 um filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Background Information
Receptors for the Fc region of IgG (Fc γ R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc γ Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized. There are three genes for human Fcγ RII /CD32 (A, B, and C) and one for mouse Fcγ RII B (CD32B). CD32 is a low affinity receptor for IgG. Low affinity immunoglobulin gamma Fc region receptor II-b (FCGR2B) also known as CD32b, FCG2, IGFR2. CD32B is expressed on B cells and myeloid dendritic cells. Ligation of CD32B on B cells down regulates antibody production and may, in some circumstances, promote apoptosis. Co-ligation of CD32B on dendritic cells inhibits maturation and blocks cell activation. CD32B may also be a target for monoclonal antibody therapy for malignancies.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants
