FGF-2/FGF-basic, human recombinant

• Catalog number
  4037-50
• Price
  Please ask
• Size
  50 ug

• Synonyms
  Prostatropin, HBGH-2, HBGF-2, FGF-2, FGF-b, Fibroblast Growth Factor
• Alternative_names
  FGF2, HBGF-2, Prostatropin, Basic fibroblast growth factor, Heparin-binding growth factor 2
• Description
  Recombinant FGF2 from E.Coli, animal origin free, ≥95% pure, biologically active, cell culture tested. A growth factor involved in angiogenesis, wound healing and embryonic development.
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Purity
  ≥95%
• Biological activity
  The ED₅₀ of human FGF-2 is determined by the dose-dependent proliferation of mouse BALB/c 3T3 cells and is typically less than 3 ng/ml.
• Molecular Weight
  Human FGF-2 is a 17.2 kDa recombinant protein
• Storage Temp
  -20°C
• Shipping
  Blue ice
• Shelf Life
  12 months
• Appearance
  Recombinant FGF-2 is available as a lyophilized powder
• Physical form description
  FGF-2 protein is lyophilized from a 1 mg/ml solution of 5 mM Tris pH 7.6 + 150 mM NaCl
• Reconstitution Instructions
  Reconstitute human FGF-2 in 5 mM Tris, pH 7.6 to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers
• Background Information
  FGF-2 (FGF2, FGF 2, FGF-basic, basic FGF, bFGF) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF-2 protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different FGF-2 isoforms have been described. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Additional description
  Basic proteins pH>7 are often produced recombinant and shouldn’t be precipitated by formic acid buffer.
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  FGF-2/FGF  
• Gene symbol
  FGF18, FGF17, NUDT6, FGF2, FGF4, FGF1, FGF13, MIR1289-2, MIR521-2, MIR509-2
• Short name
  FGF-2/FGF- , recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  FGF-2/FGF-basic, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:3674
• Gene
  FGF18
• Long gene name
  fibroblast growth factor 18
• Synonyms
  • FGF-18
  • ZFGF5
• GenBank acession
  • AB007422
• Locus
  5q35.1
• Discovery year
  1998-12-22
• Entrez gene record
  8817
• Pubmed identfication
  • 9660775
  • 9742123
• RefSeq identity
  • NM_033649
  • NM_003862
• Classification
  • Fibroblast growth factor family
• VEGA ID
  OTTHUMG00000130464

Gene info

• Identity
  HGNC:3673
• Gene
  FGF17
• Long gene name
  fibroblast growth factor 17
• Synonyms
  • FGF-13
• GenBank acession
  • AB009249
• Locus
  8p21.3
• Discovery year
  1998-12-22
• Entrez gene record
  8822
• Pubmed identfication
  • 9514906
  • 9751161
• RefSeq identity
  • NM_003867
• Classification
  • Fibroblast growth factor family
• VEGA ID
  OTTHUMG00000097051

Gene info

• Identity
  HGNC:8053
• Gene
  NUDT6
• Long gene name
  nudix hydrolase 6
• Synonyms gene name
  • nudix (nucleoside diphosphate linked moiety X)-type motif 6
• Synonyms
  • gfg-1
  • gfg
  • FGF2AS
  • FGF-AS
• GenBank acession
  • AF019632
• Locus
  4q28.1
• Discovery year
  1999-09-03
• Entrez gene record
  11162
• Pubmed identfication
  • 7984147
  • 10022609
• RefSeq identity
  • NM_007083
• Classification
  • Nudix hydrolase family
• VEGA ID
  OTTHUMG00000039507

Gene info

• Identity
  HGNC:3676
• Gene
  FGF2
• Long gene name
  fibroblast growth factor 2
• Synonyms gene
  • FGFB
• Synonyms gene name
  • fibroblast growth factor 2 (basic)
• GenBank acession
  • J04513
• Locus
  4q28.1
• Discovery year
  1986-01-01
• Entrez gene record
  2247
• Pubmed identfication
  • 9925931
• RefSeq identity
  • NM_002006
• Classification
  • Fibroblast growth factor family
  • Receptor ligands
• VEGA ID
  OTTHUMG00000039506
• Locus Specific Databases
  • LRG_1185

Gene info

• Identity
  HGNC:3682
• Gene
  FGF4
• Long gene name
  fibroblast growth factor 4
• Synonyms gene
  • HSTF1
• Synonyms gene name
  • heparin secretory transforming protein 1
• Synonyms
  • K-FGF
  • HBGF-4
  • HST
  • HST-1
  • KFGF
• Synonyms name
  • human stomach cancer, transforming factor from FGF-related oncogene
  • kaposi sarcoma oncogene
  • transforming protein KS3
• GenBank acession
  • M17446
• Locus
  11q13.3
• Discovery year
  1988-04-20
• Entrez gene record
  2249
• Pubmed identfication
  • 1611909
• RefSeq identity
  • NM_002007
• Classification
  • Fibroblast growth factor family
  • Receptor ligands
• VEGA ID
  OTTHUMG00000167887

Gene info

• Identity
  HGNC:3665
• Gene
  FGF1
• Long gene name
  fibroblast growth factor 1
• Synonyms gene
  • FGFA
• Synonyms gene name
  • fibroblast growth factor 1 (acidic)
• Synonyms
  • AFGF
  • ECGF
  • ECGFA
  • ECGFB
  • HBGF1
  • ECGF-beta
  • FGF-alpha
  • GLIO703
• Synonyms name
  • heparin-binding growth factor 1
  • endothelial cell growth factor, alpha
  • endothelial cell growth factor, beta
• GenBank acession
  • X59065
• Locus
  5q31.3
• Discovery year
  1986-01-01
• Entrez gene record
  2246
• Pubmed identfication
  • 1697263
  • 3523756
• RefSeq identity
  • NM_000800
• Classification
  • Fibroblast growth factor family
  • Receptor ligands
• VEGA ID
  OTTHUMG00000059703

Gene info

• Identity
  HGNC:3670
• Gene
  FGF13
• Long gene name
  fibroblast growth factor 13
• Synonyms gene
  • LINC00889
• Synonyms gene name
  • long intergenic non-protein coding RNA 889
• Synonyms
  • FHF2
  • FGF2
  • FLJ30672
• Synonyms name
  • fibroblast growth factor homologous factor 2
• GenBank acession
  • BC034340
• Locus
  Xq26.3-q27.1
• Discovery year
  1996-12-16
• Entrez gene record
  2258
• Pubmed identfication
  • 8790420
• RefSeq identity
  • NM_004114
• Classification
  • Fibroblast growth factor family
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000022532

Gene info

• Identity
  HGNC:35282
• Gene
  MIR1289-2
• Long gene name
  microRNA 1289-2
• Synonyms gene
  • MIRN1289-2
• Synonyms
  • hsa-mir-1289-2
• Locus
  5q31.1
• Discovery year
  2008-11-03
• Entrez gene record
  100302134
• RefSeq identity
  • NR_031621
• Classification
  • MicroRNA MIR1289 family

Gene info

• Identity
  HGNC:32113
• Gene
  MIR521-2
• Long gene name
  microRNA 521-2
• Synonyms gene
  • MIRN521-2
• Synonyms
  • hsa-mir-521-2
• Locus
  19q13.42
• Discovery year
  2005-07-15
• Entrez gene record
  574481
• RefSeq identity
  • NR_030203
• Classification
  • MicroRNA MIR515 family

Gene info

• Identity
  HGNC:33641
• Gene
  MIR509-2
• Long gene name
  microRNA 509-2
• Synonyms gene
  • MIRN509-2
• Synonyms
  • hsa-mir-509-2
• Locus
  Xq27.3
• Discovery year
  2007-10-24
• Entrez gene record
  100126301
• RefSeq identity
  • NR_030586
• Classification
  • MicroRNA MIR506 family

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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