Cell Surface Protein Isolation Kit

• Catalog number
  K295-10
• Price
  Please ask
• Size
  10 reactions

• Description
  Simple and efficient method for isolation of cell surface proteins
• Summary
  • Detection method- Western Blotting • Applications- Isolated cell surface proteins can be used for various downstream applications such as Western Blotting or other structural and functional studies
• Detection Method
  Western Blotting
• Applications
  Isolated cell surface proteins can be used for various downstream applications such as Western Blotting or other structural and functional studies
• Sample Type
  Adherent and suspension cells
• Features Benefits
  • Simple and efficient method for the isolation of cell surface proteins • Includes all required components for optimal labeling and isolation of the cell surface proteins
• Storage Conditions
  4°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Cell surface proteins play a major role in signal transduction, cell adhesion, transport and serve as diagnostic and pharmacological targets. BioVision’s Cell Surface Protein Isolation Kit provides a simple and efficient method for the isolation of cell surface proteins. In this method, cells are first labeled with Sulfo-NHS-SS-Biotin, an amine-reactive, thiol-cleavable, biotinylation reagent. Cells are subsequently lysed and the labeled cell surface proteins are isolated using Streptavidin beads. The bound proteins are then released from beads by incubating with DTT solution. Biotinylation reagent is cell-membrane-impermeable with an extended spacer arm to reduce steric hindrances associated with streptavidin binding. This convenient kit provides all the required components for optimal labeling and isolation of the cell surface proteins.
• Data Sheet Link
• Additional description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  Surface   Protein   Isolation   Kit  
• Short name
  Surface Protein Isolation Kit
• Alternative name
  cellular Surface Protein purification reagent
• Alternative technique
  kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Tissue
  cell

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