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Caspase-5, human recombinant

  • Catalog number
    1085-100
  • Product name
    Caspase-5, human recombinant
  • Size
    100 units
  • Synonyms
    Caspase-5
  • Alternative names
    Caspase-5, CASP-5, ICE(rel)-III, Protease ICH-3, Protease TY
  • Description
    A cysteine protease involved in apoptosis
  • Recombinant
    Yes
  • Source
    E. coli.
  • Purity by SDS PAGE
    ≥95%
  • Assay
    SDS-PAGE
  • Activity Specifications test method
    > 5000 units/mg
  • Unit Definition
    One unit of the recombinant caspase-5 is the enzyme activity that cleaves 1 nmol of the caspase substrate WEHD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
  • Storage Temp
    -70°C
  • Shipping
    gel pack
  • Shelf Life
    12 months
  • Appearance
    Lyophilized powder
  • Physical form description
    Lyophilized powder
  • Reconstitution Instructions
    Reconstitute to 1 unit per µl in PBS containing 15% glycerol.
  • Background Information
    Caspase-5 (also know as ICErelIII, TY) is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-5 exists in cells as an inactive proenzyme which is matured by proteolysis to yield large and small subunits. The active caspase-5 is a heterotetramer consisting of two large and two small subunits. To date the mechanism of caspase-5 activation and roles of caspase-5 in apoptosis are poorly understood. The recombinant active human caspase-5 was expressed in E. coli. The active caspase-5 is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-WEHD-pNA (Cat. #1102-200) or Ac-WEHD-AFC (1101-200).
  • Handling
    Centrifuge the vial prior to opening.
  • Usage
    For Research Use Only! Not to be used in humans
  • Datasheet
    Datasheet
  • Gene target
    Caspase-5
    • Gene info
  • Gene symbol
    CASP5
  • Short name
    Caspase-5, recombinant
  • technique filter
    • Recombinant
  • Technique
    Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
  • Alternative technique
    rec