C5a, human recombinant

• Catalog number
  4995B-1000
• Price
  Please ask
• Size
  1 mg

• Synonyms
  Recombinant human C5a
• Alternative_names
  C5a anaphylatoxin chemotactic receptor, C5a anaphylatoxin chemotactic receptor (C5a-R), (C5aR,) (CD antigen CD88), C5AR1, C5AR, C5R1
• Description
  A potent chemotactic factor for human peripheral blood neutrophils and monocytes
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Biological activity
  Human recombinant C5a is as active as that of serum derived C5a based on enzyme release assay of myeloperoxidase. The current lot of ED₅₀ = 1.36 nmolar.
• Molecular Weight
  (74 amino acids)
• Storage Temp
  -20°C
• Shipping
  Gel pack
• Shelf Life
  12 months
• Appearance
  Lyophilized protein
• Physical form description
  Lyophilized with no additives
• Reconstitution Instructions
  Reconstitute to a concentration of 1.0 mg/ml in ice-cold H₂O containing 2.5 mg/ml BSA. The solution should be aliquoted and immediately stored at -70°C for future use. Frozen/thaw will result in some loss of activity.
• Background Information
  Human complement C5a is a 74-amino acid glycopeptide generated during complement activation from the α-chain of complement C5. C5a is a potent chemotactic factor for human peripheral blood neutrophils and monocytes, and is believed to play an important role in a number of inflammatory conditions. The 74 amino acid, human C5a protein expressed in E. coli has an activity similar to that of serum derived C5a based on enzyme assay of myeloperoxidase release.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  C5a  
• Gene symbol
  C5AR1, C5, C5AR2
• Short name
  C5a, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  C5a, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:1338
• Gene
  C5AR1
• Long gene name
  complement C5a receptor 1
• Synonyms gene
  • C5R1
• Synonyms gene name
  • complement component 5 receptor 1 (C5a ligand)
  • complement component 5a receptor 1
• Synonyms
  • C5A
  • C5AR
  • CD88
• Locus
  19q13.32
• Discovery year
  1991-12-04
• Entrez gene record
  728
• Pubmed identfication
  • 1612600
• RefSeq identity
  • NM_001736
• Classification
  • Complement component GPCRs
  • Complement system regulators and receptors
  • CD molecules
• VEGA ID
  OTTHUMG00000183504

Gene info

• Identity
  HGNC:1331
• Gene
  C5
• Long gene name
  complement C5
• Synonyms gene name
  • complement component 5
• Synonyms
  • CPAMD4
  • C5a
  • C5b
• Synonyms name
  • prepro-C5
  • C5a anaphylatoxin
• GenBank acession
  • M57729
• Locus
  9q33.2
• Discovery year
  2001-06-22
• Entrez gene record
  727
• RefSeq identity
  • NM_001735
• Classification
  • C3 and PZP like, alpha-2-macroglobulin domain containing
  • Complement system activation components
  • Receptor ligands
• VEGA ID
  OTTHUMG00000020579
• Locus Specific Databases
  • C5base: Mutation registry for C5 deficiency
  • LRG_28

Gene info

• Identity
  HGNC:4527
• Gene
  C5AR2
• Long gene name
  complement C5a receptor 2
• Synonyms gene
  • GPR77
• Synonyms gene name
  • G protein-coupled receptor 77
  • complement component 5a receptor 2
• Synonyms
  • C5L2
• GenBank acession
  • AB038237
• Locus
  19q13.32
• Discovery year
  2000-01-20
• Entrez gene record
  27202
• Pubmed identfication
  • 11165367
• RefSeq identity
  • NM_018485
• Classification
  • Complement component GPCRs
• VEGA ID
  OTTHUMG00000183505

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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