Advanced Glycation End Products (AGEs) Assay Kit
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Catalog numberK929-100
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Price:
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Size100 assays
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DescriptionEasy, 1-Step assay to detect Advanced Glycation End Products
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Summary• Detection method- Fluorescence (Ex/Em= 360/460 nm) • Species reactivity- Eukaryotes
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Detection MethodFluorescence (Ex/Em 360/460 nm)
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Species ReactivityEukaryotes
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ApplicationsMeasurement of AGEs levels in Biological Fluids
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Sample Type• Serum, Urine
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Features Benefits• Simple procedure; takes less than one hour
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Storage Conditions-20°C
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Shipping Conditionsgel pack
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Shelf life12 months
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BackgroundAdvanced Glycation End Products (AGEs; also termed as glycotoxins) are a group of heterogeneous compounds formed through non-enzymatic glycation and oxidization processes between reducing sugars and protein side chains, lipids, or nucleic acids. Initial glycation and oxidation processes usually form Schiff bases and Amadori products. Glycation itself causes molecular rearrangements that lead to the generation of AGEs. The formation of these molecules is part of normal metabolism; however, under certain pathological conditions, such as oxidative stress, the production of AGEs can be abnormally excessive, leading to pathogenic conditions. The formation and accumulation of AGEs have been implicated in aging and in the development of many degenerative diseases, such as diabetes, chronic kidney disease, atherosclerosis and Alzheimer’s disease. By definition, there is not a universally accepted method to measure or quantify AGEs due to their heterogeneity. Nevertheless, since most AGEs have intrinsic fluorescence, measurements of AGE-specific fluorescence may serve as a simple and useful test to monitor circulating AGEs levels and monitor AGEs excretion. BioVision’s Advanced Glycation End Products (AGEs) Assay Kit is a 96-well microplate-based assay that can be used for the semi-quantitative estimation of fluorescent AGEs levels in Biological Fluids. The assay is based on the characteristic fluorescence (Ex/Em= 360/460 nm), a characteristic that is shared by almost all AGEs. The proprietary composition of the Assay Buffer specifically distinguishes between AGEs and non-oxidized proteins. The one-step assay uses oxidized Bovine Serum Albumin (AGE-BSA) as a Positive Control. BSA is used as Background Control and its fluorescence under assay conditions is defined as 1 relative fluorescence intensity in arbitrary units (AU or RFUSample/ RFUBackground).
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TestBiovision supplies other types of Assays as 1.
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Gene target
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Short nameAdvanced Glycation End Products (AGEs) Assay Kit
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TechniqueAssay, assays
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HostAssay
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Alternative nameAdvanced Glycation End Products (AGEs) test reagent
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Alternative techniquearrays, kits
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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