Active Cathepsin S, human recombinant

• Catalog number
  7526-500
• Price
  Please ask
• Size
  500 ug

• Synonyms
  CTSS
• Alternative_names
  CTSS
• Description
  A lysosomal cysteine protease participating in the degradation of antigenic proteins to peptides
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥90%
• Assay
  SDS-PAGE
• Biological activity
  BioVision’s Active Cathepsin S has been prepared by auto-catalytic activation of Procathepsin S (Cat # 7525) at low pH. The enzyme is fully active as seen from its ability to cleave a fluorogenic substrate Z-VVR-AFC (K144-100).
• Results
  >2000 mU/mg (1 U = Digestion of 1 µmole/min) of Ac-VVR-AFC substrate (K144-100).
• Unit Definition
  1 U = Digestion of 1 µmole/min
• Molecular Weight
  23.9 kDa (115-331 aa)
• Storage Temp
  -80°C
• Shipping
  Dry ice
• Shelf Life
  12 months
• Appearance
  Lyophilized
• Physical form description
  Lyophilized from proprietary buffer.
• Reconstitution Instructions
  Reconstitute in 50 mM sodium acetate, 100 mM NaCl (pH 5.5) to 0.1-1 mg/ml
• Background Information
  Cathepsin S (CTSS) is a lysosomal cysteine protease of the papain family and may participate in the degradation of antigenic proteins to peptides for presentation on MHC class II molecules. CTSS is synthesized as inactive precursor of 331 amino acids consisting of a 15-aa signal peptide, a propeptide of 99 aa, and a mature polypeptide of 217 aa. It is activated in the lysosomes by a proteolytic cleavage of the propeptide. The deduced amino acid sequence contains only one potential N-glycosylation site located in the propeptide. Compared with the abundant cathepsins B, L and H, cathepsin S shows a restricted tissue distribution, with highest levels in spleen, heart, and lung. In addition, evidences indicate that cathepsin S generates amyloid beta-peptide from amyloidogenic fragments of amyloid precursor protein (APP) in the endosomal/lysosomal compartment, and is implicated in the pathogenesis of Alzheimer’s disease and Down Syndrome.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Gene
  CTS protease activity also measured by zymograph electrophoresis of Cathepsins. This cathepsin is supplied in 1.
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Active   Cathepsin  
• Short name
  Active Cathepsin S, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  functionnal Cathepsin S, H. sapiens Rec.
• Alternative technique
  rec

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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