Goat Tumor Necrosis Factor Alpha (TNFa) ELISA Kit
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Catalog numberEKU07943
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Price:
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Size96 Tests
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Estimated Turnaround Time6-11 Business days
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Target NameTumor Necrosis Factor Alpha
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Target s alterntive nameDIF, TNF-A, TNFSF2, Cachectin, Tumor Necrosis Factor Ligand Superfamily Member 2
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Species ReactivityCapra hircus; Caprine (Goat)
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Sample TypeSerum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
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Detection Range15.62-1000 pg/mL
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Sensitivity5.8 pg/mL
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PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Necrosis Factor Alpha were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Necrosis Factor Alpha were tested on 3 different plates, 8 replicates in each plate.CV (%) = SD/meanX100;Intra-Assay: CV
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Detection MethodColorimetric
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Assay Time3 hours
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ApplicationFor research use only. Not for diagnostic procedures.
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Assay TypeDouble-antibody Sandwich
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Shipping ConditionsIce packs
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Storage TemperatureShort term: 4°C; Long term: see manual.
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Precaution of UseThe Stop Solution is acidic. Do not allow to contact skin or eyes.
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Shelf lifeUse Goat Tumor Necrosis Factor Alpha (TNFa) ELISA Kit before 8 months
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Tumor Necrosis Factor Alpha
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Cross ActivityNo significant cross-reactivity or interference between Tumor Necrosis Factor Alpha and analogues was observed.
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Test PrincipleNo significant cross-reactivity or interference between Tumor Necrosis Factor Alpha and analogues was observed.
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StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
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Quality SystemsThe kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
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Research AreaCytokine, Tumor immunity, Infection immunity
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DescriptionThe Goat Tumor Necrosis Factor Alpha (TNFa) ELISA Kit is a α- or alpha protein sometimes glycoprotein present in blood. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
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PropertiesE05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
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TestELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
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Latin nameCapra aegagrus hircus
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Gene target
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Gene symbolTNF
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Short nameGoat Tumor Necrosis Factor Alpha (TNFa) ELISA Kit
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TechniqueELISA kit, ELISA, Goat, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests. goats
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HostGoat, antigen assay ELISA kit 1 Kit (96 Wells), The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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SpeciesGoats
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Alternative namecaprine Tumor Necrosis Factor a (TNFa) Enzyme-linked immunosorbent assay reagent
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Alternative techniqueelisas, caprine, kits
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Tissuetumor
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Gene info
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Identity
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Gene
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Long gene nametumor necrosis factor
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Synonyms gene
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Synonyms gene name
- tumor necrosis factor (TNF superfamily, member 2)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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Classification
- Tumor necrosis factor superfamily
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VEGA ID
MeSH Data
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Name
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ConceptScope note: A cytologic technique for measuring the functional capacity of tumor stem cells by assaying their activity. It is used primarily for the in vitro testing of antineoplastic agents.
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Tree numbers
- E01.370.225.500.383.910
- E01.370.225.500.388.930
- E05.200.500.383.910
- E05.200.500.388.930
- E05.242.383.910
- E05.242.417.500
- E05.337.550.200.800
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data