Bangs Laboratories

Bangs Laboratories, Inc. is a U.S.-based biotechnology company specializing in the manufacture of microsphere-based reagents and analytical instrument calibrators. Established in 1988 and headquartered in Fishers, Indiana, Bangs Laboratories serves a global clientele in medical diagnostics, biopharma, clinical laboratories, and research institutions .

Core Products and Services

Bangs Laboratories offers a diverse range of products and services, including:

Microspheres: Polymer, silica, and magnetic microspheres used in diagnostic assays, molecular biology, and cell biology applications.
Diagnostic Reagents: Reagents for immunoassays, lateral flow tests, and turbidimetric assays.
Instrument Standards: Standards for calibrating laboratory instruments, including flow cytometers and cell counters.
Custom Services: Custom dyeing, coating, and surface modification of microspheres for specific applications.

Browse Bangs Laboratories Products

1.5mL Magnetic Separator

SKU: LS001|Size: 1 Each

40nm Gold Colloid OD1, (100mL)

SKU: GOLD40-OD1-100|Size: 100 mL

40nm Gold Colloid OD1, (25mL)

SKU: GOLD40-OD1-25|Size: 25 mL

40nm Gold Colloid OD1, (500mL)

SKU: GOLD40-OD1-500|Size: 500 mL

40nm Gold Colloid OD5, (100mL)

SKU: GOLD40-OD5-100|Size: 100 mL

40nm Gold Colloid OD5, (20mL)

SKU: GOLD40-OD5-20|Size: 20 mL

40nm Gold Colloid OD5, (5mL)

SKU: GOLD40-OD5-5|Size: 5 mL

Acridine Orange Fluorescence Reference Standard

SKU: 897-01|Size: 1 mL - 20 Tests

Alexa Fluor® 488 Fluorescence Reference Standard

SKU: 886-01|Size: 1 mL - 20 Tests

Alexa Fluor® 647 Fluorescence Reference Standard

SKU: 887-01|Size: 1 mL - 20 Tests

Allophycocyanine (APC) Fluorescence Reference Standard

SKU: 901-01|Size: 1 mL - 20 Tests

Amine Polystyrene Microspheres - 0.20µm

SKU: PA02001-01|Size: 0.5 g

Amine Polystyrene Microspheres - 0.50µm

SKU: PA03001-01|Size: 0.5 g

Amine Polystyrene Microspheres - 0.75µm

SKU: PA03002-01|Size: 0.5 g

Amine Polystyrene Microspheres - 1.0µm

SKU: PA04001-01|Size: 0.5 g

Amine Silica Microspheres (Dry) - 0.5µm

SKU: SA03000|Size: 1.0 g

Amine Silica Microspheres (Dry) - 1.0µm

SKU: SA04000|Size: 1.0 g

Amine Silica Microspheres (Dry) - 5.0µm

SKU: SA05000|Size: 1.0 g

Bangs Lab Bead Coupling Buffer, pH 4.5

SKU: BUFF1-250|Size: 250 mL

Bangs Lab Bead Coupling Buffer, pH 6.0

SKU: BUFF2-500|Size: 500 mL

Bangs Lab Bead Coupling Buffer, pH 7.4

SKU: BUFF3-2000|Size: 2000 mL

Bangs Lab Bead Coupling Buffer, pH 9.0

SKU: BUFF4-250|Size: 250 mL

Bangs Lab Bead Solution

SKU: SOLN1-1000|Size: 1000 mL

Bangs Lab Bead Storage Buffer, pH 7.4

SKU: BUFF5-250|Size: 250 mL

Bangs Lab Bead Storage Buffer, pH 8.5

SKU: BUFF6-2000|Size: 2000 mL

Bead Coupling Buffer - pH 4.5

SKU: BUFF1-01|Size: 250 mL

Bead Coupling Buffer - pH 6.0

SKU: BUFF2-01|Size: 250 mL

Bead Coupling Buffer - pH 7.4

SKU: BUFF3-01|Size: 250 mL

Bead Coupling Buffer - pH 9.0

SKU: BUFF4-01|Size: 250 mL

Bead Solution

SKU: SOLN1-01|Size: 500 mL

Bead Storage Buffer - pH 7.4

SKU: BUFF5-01|Size: 250 mL

Bead Storage Buffer - pH 8.5

SKU: BUFF6-01|Size: 250 mL

BioMag® 96-Well Plate Separator (bottom pull)

SKU: MS003|Size: 1 Each

BioMag® BioMag Amine

SKU: BM546-01|Size: 10 mL

BioMag® BioMag Carboxyl

SKU: BM570-10|Size: 10 mL

BioMag® BioMag Dextran-coated Charcoal

SKU: BM555|Size: 100 mL

BioMag® BioMag Dextran-coated Charcoal Concentrate

SKU: BM556|Size: 1000 mL

BioMag® BioMag Goat anti-Human IgG

SKU: BM563|Size: 50 mL

BioMag® BioMag Goat anti-Human IgG (Fc Specific)

SKU: BM562|Size: 50 mL

BioMag® BioMag Goat anti-Human IgM

SKU: BM561|Size: 50 mL

BioMag® BioMag Goat anti-Mouse IgG

SKU: BM549-50|Size: 50 mL

BioMag® BioMag Goat anti-Mouse IgG (Fc Specific)

SKU: BM550-50|Size: 50 mL

BioMag® BioMag Goat anti-Mouse IgM

SKU: BM558-50|Size: 50 mL

BioMag® BioMag Goat anti-Rabbit IgG

SKU: BM559-50|Size: 50 mL

BioMag® BioMag Goat anti-Rat IgG

SKU: BM560-50|Size: 50 mL

BioMag® BioMag Goat anti-Rat IgG (Fc Specific)

SKU: BM548-50|Size: 50 mL

BioMag® BioMag Protein A

SKU: BM554-2|Size: 2 mL

BioMag® BioMag Protein G

SKU: BM553-2|Size: 2 mL

BioMag® BioMag Streptavidin

SKU: BM551-5|Size: 5 mL

BioMag® BioMag Streptavidin, Nuclease-free

SKU: BM568-10|Size: 10 mL

BioMag® BioMag anti-Human CD45

SKU: BM588|Size: 5 mL

BioMag® BioMag anti-Human CD71

SKU: BM590|Size: 5 mL

BioMag® BioMag anti-Human CD8

SKU: BM583|Size: 5 mL

BioMag® BioMag anti-Mouse CD8a

SKU: BM593|Size: 5 mL

BioMag® Flask Separator

SKU: MS004|Size: 1 Each

BioMag® Magnetic Amine Immobilization Kit

SKU: BM545|Size: 1 Kit

BioMag® Maxi Amine

SKU: BMM40|Size: 10 mL

BioMag® Maxi Carboxyl

SKU: BMM30|Size: 10 mL

BioMag® Multi-6 Microcentrifuge Tube Separator

SKU: MS002|Size: 1 Each

BioMag® MultiSep Magnetic Separator

SKU: MS001|Size: 1 Each

BioMag® Oligo dT (20) - Nuclease-free

SKU: BM529|Size: 2 mL

BioMag® ProMax Albumin Removal Kit

SKU: BP658|Size: 1 Kit

BioMag® ProMax Serum IgG Removal Kit

SKU: BP659|Size: 1 Kit

BioMag® SelectaPure anti-Human CD14

SKU: BM584|Size: 5 mL

BioMag® Superparamagnetic Iron Oxide - ~10µm, Nonfunctionalized

SKU: BM547|Size: 10 mL

BioMag®Plus Amine

SKU: BP617-10|Size: 10 mL

BioMag®Plus Amine Protein Coupling Kit

SKU: BP610|Size: 1 Kit

BioMag®Plus Carboxyl

SKU: BP618|Size: 10 mL

BioMag®Plus Carboxyl Protein Coupling Kit

SKU: BP611|Size: 1 Kit

BioMag®Plus Concanavalin A

SKU: BP531-3|Size: 3 mL

BioMag®Plus Mouse anti-Fluorescein IgG

SKU: BP622|Size: 50 mL

BioMag®Plus Protein A

SKU: BP620-2|Size: 2 mL

BioMag®Plus Protein A Antibody Isolation Kit

SKU: BP614|Size: 1 Kit

BioMag®Plus Protein G

SKU: BP627-2|Size: 2 mL

BioMag®Plus Protein G Antibody Isolation Kit

SKU: BP626|Size: 1 Kit

BioMag®Plus Streptavidin

SKU: BP628|Size: 10 mL

BioMag®Plus Streptavidin / Biotin Binding Kit

SKU: BP621|Size: 1 Kit

BioMag®Plus Wheat Germ Agglutinin

SKU: BP530-3|Size: 3 mL

BioMag®Plus anti-Mouse IgG

SKU: BP619|Size: 50 mL

BioMag®Plus anti-Mouse IgG Antibody Coupling Kit

SKU: BP612|Size: 1 Kit

Biotin Coated Polymer Microspheres - 10µm

SKU: CP10000-01|Size: 1 mL

COMPEL™ Fluorescent Uniform Magnetic Microspheres, Dragon Green - 3µm

SKU: UMDG001-01|Size: 1 mL

COMPEL™ Fluorescent Uniform Magnetic Microspheres, Dragon Green - 6µm

SKU: UMDG002-01|Size: 1 mL

COMPEL™ Fluorescent Uniform Magnetic Microspheres, Dragon Green - 8µm

SKU: UMDG003-01|Size: 1 mL

COMPEL™ Fluorescent Uniform Magnetic Microspheres, Envy Green - 3µm

SKU: UMEG001-01|Size: 1 mL

COMPEL™ Fluorescent Uniform Magnetic Microspheres, Flash Red - 3µm

SKU: UMFR001-01|Size: 1 mL

COMPEL™ Fluorescent Uniform Magnetic Microspheres, Flash Red - 6µm

SKU: UMFR002-01|Size: 1 mL

COMPEL™ Fluorescent Uniform Magnetic Microspheres, Flash Red - 8µm

SKU: UMFR003-01|Size: 1 mL

COMPEL™ Fluorescent Uniform Magnetic Microspheres, Glacial Blue - 3µm

SKU: UMGB001-01|Size: 1 mL

COMPEL™ Fluorescent Uniform Magnetic Microspheres, Glacial Blue - 6µm

SKU: UMGB002-01|Size: 1 mL

COMPEL™ Fluorescent Uniform Magnetic Microspheres, Glacial Blue - 8µm

SKU: UMGB003-01|Size: 1 mL

COMPEL™ Uniform Magnetic Microspheres, COOH - 3µm

SKU: UMC3001-01|Size: 0.5 g

COMPEL™ Uniform Magnetic Microspheres, COOH - 6µm

SKU: UMC3002-01|Size: 0.5 g

COMPEL™ Uniform Magnetic Microspheres, COOH - 8µm

SKU: UMC4001-01|Size: 0.5 g

COMPEL™ Uniform Magnetic Microspheres, Streptavidin - 3µm

SKU: UMC0100-01|Size: 1 mL

COMPEL™ Uniform Magnetic Microspheres, Streptavidin - 6µm

SKU: UMC0101-01|Size: 1 mL

COMPEL™ Uniform Magnetic Microspheres, Streptavidin - 8µm

SKU: UMC0102-01|Size: 1 mL

Carboxyl Magnetic Sampler Pack

SKU: 21940|Size: 1 Pack

Carboxyl Polystyrene Microspheres - 0.025µm

SKU: PC02001-01|Size: 0.5 g

Carboxyl Polystyrene Microspheres - 0.050µm

SKU: PC02002-01|Size: 0.5 g

Recent Breakthroughs

Metabolomic insights into Lyme neuroborreliosis: Exploring cerebrospinal fluid for diagnostic clues.

Kuukkanen I, Pietikäinen A, Rissanen T, Hurme S, Kortela E, Hytönen J, Karonen M.

Ticks Tick Borne Dis

PMID:42001681

Free PMC article

Lyme neuroborreliosis (LNB), a disseminated manifestation of Lyme borreliosis (LB), arises when Borrelia burgdorferi sensu lato (Bbsl) spirochetes disseminate within the host and damage the peripheral nervous system and meninges, and in rare cases, also the parenchyma of the central nervous system (CNS). While early-stage LB is diagnosed clinically, accurate diagnosis of LNB requires cerebrospinal fluid (CSF) analysis, demonstrating pleocytosis and intrathecal synthesis of Bbsl-specific antibodies. There are, however, limitations in current LNB diagnostics, such as the unspecific nature of pleocytosis and post-treatment persistence of intrathecal antibodies necessitating the search for novel biomarkers. In this study, we employed untargeted ultrahigh-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) to profile small metabolites (<1500 Da) in CSF samples from subjects with definite acute LNB. Comparative analyses of metabolite profiles were performed between pretreatment subject samples (n = 63) and the following groups: (A) a subset of samples collected three weeks after treatment initiation from the same individuals (n = 36), (B) Bbsl antibody-negative subjects (non-LNB, n = 61), (C) subjects with other CNS infections (n = 21). Additionally, pretreatment LNB samples were compared between individuals with radiculitis (n = 40) and those without radiculitis (n = 23) (D). Out of 4222 molecular features (MFs) detected, 131 were prioritized based on statistical significance and magnitude of change for further detailed structural characterization. Altered metabolite classes included compounds from lysophospholipids [e.g., lysophosphatidylcholine (16:0), and lysophosphatidylethanolamine (18:0)], sphingomyelins [e.g., sphingomyelin (d18:1/14:0) and sphingomyelin d16:1/16:0)], sphingoid bases (e.g., d19:0 sphinganine, and 3-ketosphingosine), fatty acid amides (e.g., palmitoleamide and oleamide), cyclic phosphatidic acids [i.e., cyclic phosphatidic acid (16:0) and cyclic phosphatidic acid (18:2)], and amino acid metabolism (i.e., DL-glutamine, 5-hydroxytryptophan and DL-kynurenine). These findings underscore the potential of CSF metabolomics as a powerful complementary tool for diagnosing LNB and differentiating it from other CNS conditions. The identified metabolic signatures offer a foundation for future biomarker development and may enhance diagnostic precision, guide treatment strategies, and deepen our understanding of LNB pathogenesis.

Isotope Dilution NanoLC-MS/MS Quantitation of Methylglyoxal DNA-Protein Cross-Links: Formation and Repair in Human Cells.

Omondi RO, Barnes EM, Gurajala KC, Fisette G, Chaudray IA, Erber L.

Anal Chem

PMID:41995195

Free PMC article

DNA-protein cross-links (DPCs) represent a prevalent form of DNA damage that forms when cellular proteins become covalently trapped to DNA strands upon exposure to various endogenous and exogenous agents. Methylglyoxal is an endogenous metabolite that reacts with guanine and adenine bases in DNA and RNA, as well as cysteine, arginine, and lysine residues in proteins, generating advanced glycation end-products (AGEs), including DPCs. These modifications have been linked to human disease, including cancer, liver disease, diabetes, and neurodegenerative disorders. Herein, we present a mass spectrometry method for quantifying MGO-induced DNA-protein cross-links (DPCs) in human cells. We prepared an isotope 15 N 2 13 C 6 -dG-MGO-Lys internal standard and developed a quantitative liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for detecting and quantifying the formation and repair of dG-MGO-Lys DPCs in cells. Genomic DNA was extracted, subjected to sequential protease and nuclease digestion, purified by offline high-performance liquid chromatography (HPLC), and analyzed by LC-MS/MS. The method's standard curve showed a strong linear relationship across a concentration range of 10-1000 fmol ( R 2 = 0.9994). The method achieved limits of detection (LOD) and quantification (LOQ) of 10 and 20 fmol, respectively. Inhibition of proteasome and SPRTN activity revealed that SPRTN functions as a predominant proteolytic enzyme in MGO DPC repair. Overall, this analytical approach can offer valuable insights into the relevance of DPCs in diseases linked to elevated MGO levels.

Label-Free High-Throughput Screening of CYP3A4 Inhibitors Using Acoustic Ejection Mass Spectrometry.

Rimmer MA, Wang J, Ranathunge TA, Shi Z, Li Y, Lee D, Chai SC, Lin W, Twarog NR, Ansari AZ, Shelat AA, Young BM, Chen T, Yang L.

Anal Chem

PMID:41997578

Free PMC article

Label-free high-throughput screening (HTS) technologies have greatly accelerated early drug discovery by allowing direct measurement of biochemical activity without the need for chemical labels. Techniques like MALDI-TOF, RapidFire, desorption electrospray ionization, and acoustic-mist ionization mass spectrometry have shown strong analytical performance. More recently, acoustic droplet ejection mass spectrometry (AEMS) has emerged as a next-generation platform capable of analyzing over a thousand data points per hour. In this study, we thoroughly evaluated an AEMS workflow using cytochrome P450 3A4 (CYP3A4) as a model enzyme. Screening a library of 9702 compounds, this work represents the first successful label-free HTS campaign for CYP3A4 inhibitors in a 384-well format using AEMS. The result using nifedipine as a substrate was comparable to the widely used P450-Glo luminescence assay but with fewer inhibitor hits identifications. To further compare and parse differences between the methods and the substrates, 819 hits were retested using AEMS with luciferin isopropyl acetal, the same substrate used in the luminescence assay. The findings confirmed that both the assay method and the substrate influence which inhibitors were detected. Overall, this study established AEMS as a robust and reliable tool for CYP3A4 inhibitor screening. It offers high analytical precision, fewer false positives, compatibility with multiple substrates, and direct detection of metabolites, providing a practical way to validate hits and improve confidence in early drug discovery efforts.

Quantification of the Active Metabolites 4-Methylaminoantipyrine and 4-Aminoantipyrine of Dipyrone From Human Plasma by LC-MS/MS.

Schmidt S, Ihmsen H, Golditz T, Schüttler J, Wehrfritz A.

Int J Anal Chem

PMID:42004449

Free PMC article

Dipyrone (metamizole) is widely used as a nonopioid analgesic in perioperative and intensive care settings; however, its administration may be associated with severe adverse effects. In this study, we describe an analytical assay for the quantification of the active metabolites 4-methylaminoantipyrine (4-MAA) and 4-aminoantipyrine (4-AA) in human plasma using ultra-high-performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS). In addition, the protein-unbound fractions of both metabolites were determined by ultrafiltration. Chromatographic separation was performed on a UPLC system using gradient elution, followed by MS/MS detection with an electrospray ionization source. The limits of detection for both metabolites were 100 ng/mL. Across the investigated concentration range (100-10,000 ng/mL), the relative error (%RE) ranged from -6.3% to +3.5%. Intra-day and inter-assay variability were below 10%. Method validation was conducted in accordance with the 2018 FDA Bioanalytical Method Validation Guidance, and all evaluated parameters met the required criteria for analytical accuracy and precision. The developed assay is suitable for monitoring dipyrone metabolites and may support the prevention of potential overdosing during prolonged analgesic therapy or in intensive care settings.

Metabolomics profiling of acute myelogenous leukemia patients to identify systemic differences associated with in vitro sensitivity to SYK inhibitors.

Brattås MK, Hatfield KJ, Paulsen Rye K, Reikvam H.

Metabolomics

PMID:41995902

Free PMC article

Introduction Acute myelogenous leukemia (AML) is a heterogeneous hematological malignancy. Despite therapeutic advances, high relapse rates and poor survival persist, underscoring the need for new treatment strategies. Spleen tyrosine kinase (SYK) is crucial for leukemic cell survival and proliferation, and SYK inhibition is being explored in AML therapy. Objective This study aimed to identify whether distinct baseline serum metabolic profiles can differentiate between AML patient samples exhibiting high versus low in vitro sensitivity to SYK inhibition. Methods Leukemic cells from 49 AML patients were evaluated for their in vitro antiproliferative response to SYK-selective inhibitors (entospletinib, RO9021) and dual SYK/FLT3 inhibitors (fostamatinib, TAK-659). Serum samples from the same patients were subjected to untargeted metabolomic profiling using liquid chromatography-mass spectrometry (LC-MS). Bioinformatic analyses were applied to identify metabolic signatures associated with differential drug sensitivity. Results Hierarchical clustering identified three drug sensitivity groups, i.e. high, intermediate, low. Untargeted metabolomics identified 1204 metabolites across major pathways. Comparative analyses revealed distinct metabolic differences between patients with high versus low sensitivity to SYK inhibitors. For dual SYK/FLT3 inhibitors, lipid metabolites consistently were more discriminative for drug response, while for SYK selective inhibitors the profiles were more heterogeneous. Pathway analyses highlighted alterations in amino acid, lipid, and xenobiotic metabolism, including arginine biosynthesis and glycine/serine metabolism, as potential determinators for effect of SYK inhibition. Conclusion This study highlights potential metabolite signatures associated with in vitro SYK-targeted therapy in AML. These findings support future research to identify potential serum biomarkers, guiding personalized SYK inhibition strategies in AML.