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Available ordering format
Liquid
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Immunogen
to be determined
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified IgG
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
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Verified applications
Contact Gentaur support center
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Connected products
other TRITC conjugated secondary antibodies to mouse immunoglobulins
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Recommended dilutions for use
Needs to be determined by end user for immunocytochemistry (ICC), immunohistochemistry ( IHC) and for flow cytometry.
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
to be determined
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Possible reactivity
to be determined
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No reactivity
to be determined
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Supplementary information
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References
Refer to PubMed
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Scientific context
Rabbit anti-mouse IgG (H&L), Rhodamine (TRITC - tetramethylrhodamine-5-isothiocyanate) conjugated, is a secondary antibody which binds to all mouse IgGs in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibody is adsorbed against human serum and affinity purified using solid phase mouse IgG.
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Notes
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins, or human serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Rabbits are used for polyclonal antibody production by agisera. Rabbit antibodies are very stable and can be stored for several days at room temperature. agisera adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Serum should be stored at +5°C
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Latin name
Oryctolagus cuniculus
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Description
Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
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Group
Sera
