- Catalog numberAS10 1461
- Product nameGoat, L), HRP conjugated, min. cross-reactivity to human serum
- Size1 mg
- PriceAsk For Price
- Available ordering formatLyophilized
- ImmunogenPurified rabbit IgG (H&L)
- Raised inGoat
- ClonalityPolyclonal
- ClonePolyclonal
- PurificationAffinity purified IgG
- How to reconstituteFor reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
- Storage conditionStore lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
- Verified applicationsContact Gentaur support center
- Connected productsother HRP conjugated secondary antibodies against rabbit IgG (H&L)
- Recommended dilutions for useThis conjugate is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:500-1:5 000 for Immunohistochemistry 1:200-1:5000 for ELISA/Western blot
- Molecular weight expected аpparentsee datasheet
- Verified reactivityto be determined
- Possible reactivityto be determined
- No reactivityto be determined
- Supplementary informationContact Gentaur support center at [email protected]
- ReferencesRefer to PubMed
- Scientific contextGoat anti-rabbit IgG (H&L) is a secondary antibody conjugated to HRP, which binds to rabbit IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rabbit IgG (H&L).
- NotesPurity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml. Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.1 % (v/v) Kathon CG as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rabbit IgG, light chains on all rabbit immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rabbit serum proteins, human serum proteins.
- Protein numberRefer to NCBI
- TAIR numberRefer to NCBI
- Hyperlink
- Gene targetL conjugated min cross
- HostGoat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
- Short nameGoat, L), conjugated, min. cross-reactivity serum
- LabelHRP
- label filter
- HRP
- technique filter
- serum
- Goat
- Techniqueserum, Goat, antibody Conjugates, goats
- Alternative techniquesera, caprine
- Tissueserum
- tissue filter
- serum