Goat, L), HRP conjugated

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• Catalog number
  AS09 603
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• Size
  1 mg

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• Available ordering format
  Lyophilized
• Immunogen
  Purified chicken IgY, whole molecule.
• Raised in
  Goat
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Affinity purified goat IgG, using solid phase chicken IgY.
• How to reconstitute
  For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
• Storage condition
  Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
• Verified applications
  ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
• Connected products
  AS10 1489 | Rabbit anti-Chicken IgY (H&L), HRP conjugatedAS09 606 | Goat anti-hen IgY (H&L) AP conjugatedAS09 610 | Goat anti-hen IgY (H&L) biotin conjugated
• Recommended dilutions for use
  1: 10 000 -1: 150 000 (ELISA), 1: 500 -1: 5000 (IHC), 1 : 20 000 with enhanced ECL and 1: 10 000 with standard ECL (WB)
• Molecular weight expected аpparent
  see datasheet
• Verified reactivity
  Chicken IgY heavy and light chains (H&L).
• Possible reactivity
  to be determined
• No reactivity
  no confirmed exceptions from predicted reactivity known in the moment
• Supplementary information
  Antibody binds to:heavy chains on chicken IgYlight chains on all chicken immunoglobulinsNo reactivity is observed to non-immunoglobulin chicken serum proteins based in immunoelectrophoresis.Chicken immunoglobulin is often called hen or chicken IgG, however it is derived from egg yolk, therefore IgY.
• References
  Huang et al. (2015). Effects of exogenous salicylic acid on the physiological characteristics of Dendrobium officinale under chilling stress. Plant Growth Regulation pp 1-10.
• Scientific context
  Goat anti-chicken IgY (H&L) is a secondary antibody conjugated to HRP (horse radish peroxidase) which binds to chicken IgY in immunological assays.
• Notes
  This HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free 0.1 % (v/v) of Kathon CG is used as preservative.
• Protein number
  Refer to NCBI
• TAIR number
  Refer to NCBI
• Hyperlink
• Latin name
  Capra aegagrus hircus
• Properties
  HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
• Conjugation
  HRP Conjugated

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• Gene target
  conjugated  
• Short name
  Goat, L), conjugated
• Technique
  Goat, antibody Conjugates, goats
• Host
  Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
• Label
  HRP
• Species
  Goats
• Alternative name
  caprine, L), horseradish peroxidase coupled
• Alternative technique
  caprine

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