Goat, L), HRP conjugated
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Catalog numberAS09 603
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Price:
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Size1 mg
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Available ordering formatLyophilized
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ImmunogenPurified chicken IgY, whole molecule.
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Raised inGoat
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ClonalityPolyclonal
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ClonePolyclonal
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PurificationAffinity purified goat IgG, using solid phase chicken IgY.
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How to reconstituteFor reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
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Storage conditionStore lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
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Verified applicationsELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
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Connected productsAS10 1489 | Rabbit anti-Chicken IgY (H&L), HRP conjugatedAS09 606 | Goat anti-hen IgY (H&L) AP conjugatedAS09 610 | Goat anti-hen IgY (H&L) biotin conjugated
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Recommended dilutions for use1: 10 000 -1: 150 000 (ELISA), 1: 500 -1: 5000 (IHC), 1 : 20 000 with enhanced ECL and 1: 10 000 with standard ECL (WB)
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Molecular weight expected аpparentsee datasheet
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Verified reactivityChicken IgY heavy and light chains (H&L).
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Possible reactivityto be determined
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No reactivityno confirmed exceptions from predicted reactivity known in the moment
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Supplementary informationAntibody binds to:heavy chains on chicken IgYlight chains on all chicken immunoglobulinsNo reactivity is observed to non-immunoglobulin chicken serum proteins based in immunoelectrophoresis.Chicken immunoglobulin is often called hen or chicken IgG, however it is derived from egg yolk, therefore IgY.
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ReferencesHuang et al. (2015). Effects of exogenous salicylic acid on the physiological characteristics of Dendrobium officinale under chilling stress. Plant Growth Regulation pp 1-10.
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Scientific contextGoat anti-chicken IgY (H&L) is a secondary antibody conjugated to HRP (horse radish peroxidase) which binds to chicken IgY in immunological assays.
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NotesThis HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free 0.1 % (v/v) of Kathon CG is used as preservative.
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Protein numberRefer to NCBI
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TAIR numberRefer to NCBI
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Latin nameCapra aegagrus hircus
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PropertiesHRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
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ConjugationHRP Conjugated
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Gene target
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Short nameGoat, L), conjugated
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TechniqueGoat, antibody Conjugates, goats
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HostGoat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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LabelHRP
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SpeciesGoats
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Alternative namecaprine, L), horseradish peroxidase coupled
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Alternative techniquecaprine
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