Goat, F(ab)'2 fragment, HRP conjugated
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Catalog numberAS10 1039
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Price:
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Size0.5 mg
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Available ordering formatLyophilized
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Immunogento be determined
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Raised inGoat
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ClonalityPolyclonal
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ClonePolyclonal
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PurificationAffinity purified IgG, F(ab)'2 fragment
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How to reconstituteFor reconstitution please add 0.55 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
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Storage conditionStore lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
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Verified applicationsContact Gentaur support center
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Connected productsother HRP conjugated secondary antibodies to human immunoglobulins
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Recommended dilutions for use1:500-1: 5000 for immunohistochemistry (IHC), 1:10 000-1:50 000 for ELISA/western blot.
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Molecular weight expected аpparentsee datasheet
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Verified reactivityto be determined
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Possible reactivityto be determined
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No reactivityto be determined
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Supplementary informationContact Gentaur support center at [email protected]
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ReferencesRefer to PubMed
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Scientific contextGoat anti-human IgG + IgA + IgM, HRP conjugated is a F(ab)'2 fragment, of a secondary antibody which binds to human IgG+IgA+IgM in immunological assays. Antibody purity is > 90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
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NotesThis antibody reacts with the heavy chains on human IgG + IgA + IgM, and the light chains on all human immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative.
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Protein numberRefer to NCBI
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TAIR numberRefer to NCBI
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Latin nameCapra aegagrus hircus
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PropertiesHRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
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ConjugationHRP Conjugated
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Gene target
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Short nameGoat, F(ab)'2 , conjugated
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TechniqueGoat, antibody Conjugates, goats
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HostGoat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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IsotypeAntibody fragments and scFv fragments, single chain variable fragments can be E. coli expressed. Fragment peptides for polyclonals give monoclonal like antibodies as the epitope is very small. IgG Fc and(ab)2 fragments are small and stable subunits of the antibody obtained by enzymatic digestion. Complement fragments, bp DNA fragments are common.
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LabelHRP
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SpeciesGoats
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Alternative namecaprine, F(Antibody)'2 fragment, horseradish peroxidase coupled
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Alternative techniquecaprine
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