Goat, F(ab)'2 fragment, HRP conjugated

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• Catalog number
  AS10 1039
• Price:

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• Size
  0.5 mg

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• Available ordering format
  Lyophilized
• Immunogen
  to be determined
• Raised in
  Goat
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Affinity purified IgG, F(ab)'2 fragment
• How to reconstitute
  For reconstitution please add 0.55 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
• Storage condition
  Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
• Verified applications
  Contact Gentaur support center
• Connected products
  other HRP conjugated secondary antibodies to human immunoglobulins
• Recommended dilutions for use
  1:500-1: 5000 for immunohistochemistry (IHC), 1:10 000-1:50 000 for ELISA/western blot.
• Molecular weight expected аpparent
  see datasheet
• Verified reactivity
  to be determined
• Possible reactivity
  to be determined
• No reactivity
  to be determined
• Supplementary information
  Contact Gentaur support center at [email protected]
• References
  Refer to PubMed
• Scientific context
  Goat anti-human IgG + IgA + IgM, HRP conjugated is a F(ab)'2 fragment, of a secondary antibody which binds to human IgG+IgA+IgM in immunological assays. Antibody purity is > 90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
• Notes
  This antibody reacts with the heavy chains on human IgG + IgA + IgM, and the light chains on all human immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative.
• Protein number
  Refer to NCBI
• TAIR number
  Refer to NCBI
• Hyperlink
• Latin name
  Capra aegagrus hircus
• Properties
  HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
• Conjugation
  HRP Conjugated

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• Gene target
  conjugated  
• Short name
  Goat, F(ab)'2 , conjugated
• Technique
  Goat, antibody Conjugates, goats
• Host
  Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
• Isotype
  Antibody fragments and scFv fragments, single chain variable fragments can be E. coli expressed. Fragment peptides for polyclonals give monoclonal like antibodies as the epitope is very small. IgG Fc and(ab)2 fragments are small and stable subunits of the antibody obtained by enzymatic digestion. Complement fragments, bp DNA fragments are common.
• Label
  HRP
• Species
  Goats
• Alternative name
  caprine, F(Antibody)'2 fragment, horseradish peroxidase coupled
• Alternative technique
  caprine

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