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Available ordering format
Liquid
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Immunogen
to be determined
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Raised in
Goat
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified IgG, F(ab)'2 fragment
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
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Verified applications
Contact Gentaur support center
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Connected products
other fluorescent dye conjugated secondary antibodies to human IgG
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Recommended dilutions for use
1:20-1: 2000 for immunohistochemistry (IHC), 1:50-1: 5000 for immunocytochemistry (ICC).
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
to be determined
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Possible reactivity
to be determined
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No reactivity
to be determined
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Supplementary information
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References
Refer to PubMed
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Scientific context
Goat anti-human IgG Fc, F(ab)'2 fragment, FITC (fluorescein-5-isothiocyanate) conjugated, is a secondary antibody which binds to human IgG Fc constant part in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm.Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are adsorbed against bovine,mouse,rabbit serum with a purify of >90% based on SDS-PAGE. Small amounts of intact IgG may be present.
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Notes
This antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.Minimum cross-reactivity is observed with the light chains on human immunoglobulins or to non-immunoglobulin human serum proteins based on immunoelectrophoresis. No reactivity is observed to bovine, mouse or rabbit serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 0.05 % (w/v) sodium azide as preservative. 1% (w/v) BSA, Protease/IgG free.
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Properties
This agisera Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. agisera FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Latin name
Capra aegagrus hircus, Mus musculus, Oryctolagus cuniculus
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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About
Rabbits are used for polyclonal antibody production by agisera. Rabbit antibodies are very stable and can be stored for several days at room temperature. agisera adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Serum should be stored at +5°C
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Description
Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
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Group
Sera
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Gene target
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Short name
Goat, F(ab)'2 , FITC conjugated, min. cross-reactivity ,mouse rabbit serum
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Technique
Rabbit, Mouse, serum, FITC, Goat, antibody Conjugates, Fluorescein, goats, mouses
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial. Rabbits
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Isotype
Antibody fragments and scFv fragments, single chain variable fragments can be E. coli expressed. Fragment peptides for polyclonals give monoclonal like antibodies as the epitope is very small. IgG Fc and(ab)2 fragments are small and stable subunits of the antibody obtained by enzymatic digestion. Complement fragments, bp DNA fragments are common.
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Label
FITC
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Species
Mouse, Bos Taurus genes are available form the Bovine Genome database. Goats, Mouses
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Alternative name
caprine, F(Antibody)'2 fragment, fluorecein coupled, min. cross-This (antibody to-) recognizes to bovine,mouse,production species: rabbit blood serum
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Alternative technique
rabbit-anti, sera, murine, caprine, fluorescine
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Tissue
serum
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