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Available ordering format
Lyophilized
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Immunogen
Recombinant CpxA (residues 26 to 168) over-expressed as a N-terminal fusion with His(x6)-tag, which was removed prior to immunization. The antigen originates from enteropathogenic Yersinia pseudotuberculosis YPIII; B1JQV1, NCBI annotated locus tag YPK_4133.
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
For reconstitution add 50 µl of sterile water.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB)
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Connected products
Plant protein extraction buffer Secondary antibodies
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Recommended dilutions for use
1 : 2000 with standard ECL (WB)
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Molecular weight expected аpparent
51.8 | 52-53 kDa
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Verified reactivity
Yersinia pseudotuberculosis
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Possible reactivity
Yersinia pestis, Yersinia enterocolica and related species
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No reactivity
no confirmed exceptions from predicted reactivity are currently known
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Supplementary information
to be added when available
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References
to be added when available, antibody released in April 2013
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Scientific context
CpxA (Conjugative Plasmid Expression) is the 458 amino acid protein, a bacterial inner membrane histidine protein kinase, responsible for sensing misfolded proteins in the periplasm. It is part of CpxA/CpxR two component signal transduction system, where CpxR is the cognate response regulator. In sensing bacterial envelope stress, the Cpx pathway regulates the production of a number of periplasmic folding and trafficking factors to maintain outer membrane integrity. It is also responsible for global control of virulence factor expression. Alternative name: Integral membrane sensor signal transduction histidine kinase.
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Notes
The strains were grown in LB broth until late stationary phase (absorbance at 600nm of ~1.5). To avoid interference from cross-reacting bands, aim to maximize protein separation with the region of 35 to 55 kDa. CpxA could be routinely detected from a 20 µL of sample that was derived from 1ml of pelleted bacteria resuspended in 200 µl of 1x loading buffer. Re-use of diluted working strength antibody is not recommended.
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Kit
Plasmid mini made and maxi DNA purification kits can be silica gel or anion exchange, endotoxin free and are used to produce pure plasmids that are small DNA molecules within a cell separated from chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.
