Multi Fusion-Tagged recombinant Protein 58-Kda containing 17-tags (T-7, HSV, C-myc, VSV-G, Glu-Glu, V5, e-tag, GST, Flag, S-tag, HA, KT3, E2, Au1, Au5, 6xHis-tags) for ELISA
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Catalog numberMFPM53-R-40
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Price:
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Size40 ug
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Stock availabilityAvailable
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CategoryRecombinant Protein
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Antibody typeN/A
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Antibody hostN/A
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Antibody conjugateHis-Tag
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Technical datasheetContact Gentaur to request the datasheet or ask our specialists for more information.
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NotesThe Multi Fusion-Tagged recombinant Protein 58-Kda containing 17-tags (T-7, HSV, C-myc, VSV-G, Glu-Glu, V5, e-tag, GST, Flag, S-tag, HA, KT3, E2, Au1, Au5, 6xHis-tags) for ELISA is manufactured for Research Use Only or for diagnostics purposes.
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ConjugatedThe polyhistidine (6xHis) tag or Histidine Epitope Tag (HIS Tag, 6XHIS Tag with Histidine (abbreviated as His or H; encoded by the codons CAU and CAC) is an α-amino acid that is used in the biosynthesis of proteins. It is the most used tag for purification by Affinity Chromatography (IMAC). The pentadentate chelator is the best chelator in Immobilized Metal ion Affinity chromatography (IMAC) for the6xhis-tagged proteins.
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PropertiesE05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
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TestELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
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ConjugationFlag
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DescriptionFusion proteins or chimeric proteins are proteins created through the joining of two or more genes that originally coded for separate proteins. A GFP gene is often used as tag to a reporter gene. Fusion lentiverctors can be used as viral particles to produce proteins that carry for example a GFP tag. Antigen purification of recombinant fusion tag proteins is a frequent strategy using a Fralg tag. The kilo Daltons subunit weight of Multi -Tagged recombinant Protein 58- 17-tags (T-7, HSV, C-myc, VSV-G, Glu-Glu, V5, e-tag, GST, , S-tag, HA, KT3, E2, Au1, Au5, -tags) for ELISA compared to your protein ladder can be shifted a little due to electrophoresis effects. 1 kDa = 1000 g/mol protein
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SourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolIGHV3-71, GALNT7, MGST3, PIRC95, PIRC91, PIRC90, PIRC93, PIRC92, SNORD115-17
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Short nameMulti -Tagged recombinant Protein 58- 17-tags (T-7, HSV, C-myc, VSV-G, Glu-Glu, V5, e-tag, GST, , S-tag, HA, KT3, E2, Au1, Au5, -tags) for ELISA
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TechniqueRecombinant, ELISA, multi, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. multi, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. adi advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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Hostantigen assay ELISA kit 1 Kit (96 Wells)
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Labeltag
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SpeciesAll species
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Alternative nameMulti Fusion-Tagged Rec. Protein 58-kiloDalton containing 17-tags (T-7, HSV, C-v-myc myelocytomatosis viral oncogene homolog (avian), VSV-G, L-gutamic acid-L-gutamic acid, V5, e-detection labelled, GST, Flag, S-detection labelled, HA, KT3, E2, Au1, Au5, 6xHis-tags) to measure Enzyme-linked immunosorbent assay
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Alternative techniquerec, elisas
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Alternative to gene targetv-myc myelocytomatosis viral oncogene homolog (avian), bHLHe39 and c-Myc and MRTL and MYCC, MYC and IDBG-35442 and ENSG00000136997 and 4609, E-box binding, nuclei, Bmyc and IDBG-147082 and ENSMUSG00000049086 and 107771, MYC and IDBG-644147 and ENSBTAG00000008409 and 511077
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Virushsv
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Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3-71 (pseudogene)
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Synonyms gene
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Synonyms gene name
- immunoglobulin heavy variable 3-71
- immunoglobulin heavy variable 3-G pseudogene (provisional)
- immunoglobulin heavy variable 3-G (provisional)
- immunoglobulin heavy variable 3-71 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namepolypeptide N-acetylgalactosaminyltransferase 7
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Synonyms gene name
- UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 7 (GalNAc-T7)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-09-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Polypeptide N-acetylgalactosaminyltransferases
- MicroRNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicrosomal glutathione S-transferase 3
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1997-10-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Microsomal glutathione S-transferases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 95
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 91
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 90
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 93
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 92
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-17
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
MeSH Data
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Name
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ConceptScope note: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
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Tree numbers
- E05.478.566.350.170
- E05.478.566.380.360
- E05.478.583.400.170
- E05.601.470.350.170
- E05.601.470.380.360
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Qualifiersethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data