Kappa FITC/ Lambda-PE/ CD19-PECy5, Mouse Monoclonal antibody-Human, Clone: Polyclonal/ Polyclonal/ J4.119
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Catalog numberACYTKF2LPE19C2
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Price:
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Size1.5 ml
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Alternative name 1Kappa FITC/ Lambda-PE/ CD19-PECy5, Mab anti-Human, Clone: Polyclonal/ Polyclonal/ J4.119
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Alternative name 2Kappa FITC/ Lambda-PE/ CD19-PECy5, Mab antibody-Human, Clone: Polyclonal/ Polyclonal/ J4.119
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Alternative name 3Kappa FITC/ Lambda-PE/ CD19-PECy5, Mab antibody-Homo sapiens, Clone: Polyclonal/ Polyclonal/ J4.119
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Alternative name 4anti-KappaLambda-CD19- Clone: Polyclonal/ Polyclonal/ J4.119
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Alternative name 5KappaLambda-CD19- Clone: Polyclonal/ Polyclonal/ J4.119
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ClonalityMonoclonal Antibody
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DescriptionThis is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
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Storage and handlingGenerally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
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AdvisoryTo keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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PropertiesIf you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This accurate-monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. accurate-monoclonals FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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ConjugationAnti-FITC Antibody
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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GroupPolyclonals and antibodies
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French translationanticorps
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Gene target
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Gene symbolCD19, ETV3, ERF
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Short nameKappa FITC/ Lambda-PE/ CD19-PECy5, Mouse Monoclonal antibody- , Clone: Polyclonal/ Polyclonal/ J4 119
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TechniqueMouse monoclonal, Polyclonal, Antibody, Mouse, FITC, antibodies against human proteins, antibodies for, Fluorescein, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Hostmouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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LabelFITC
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SpeciesHuman, Humans, Mouses
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Alternative nameKappa fluorecein/ Lambda-peroxidase/ CD19 molecule-PECy5, Mouse monoclonal (antibody to-)-H. sapiens, clonality: polyclonal/ polyclonal/ J4.119
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Alternative techniquepolyclonals, antibodies, murine, fluorescine
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Alternative to gene targetCD19 molecule, B4 and CVID3, CD19 and IDBG-23791 and ENSG00000177455 and 930, protein binding, Cell surfaces, Cd19 and IDBG-209475 and ENSMUSG00000030724 and 12478, CD19 and IDBG-638894 and ENSBTAG00000032122 and
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Gene info
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Identity
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Gene
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Long gene nameCD19 molecule
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Synonyms gene name
- CD19 antigen
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Locus
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Discovery year1991-06-04
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Entrez gene record
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Classification
- CD molecules
- Immunoglobulin like domain containing
- Minor histocompatibility antigens
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameETS variant transcription factor 3
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Synonyms gene name
- ets variant gene 3, ETS family transcriptional repressor
- ETS variant 3
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Synonyms
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GenBank acession
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Locus
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Discovery year1994-12-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- ETS transcription factor family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameETS2 repressor factor
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Synonyms gene name
- Ets2 repressor factor
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-07-17
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- ETS transcription factor family
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data