CD134, OX-40 Ligand, CD4 +'ve activated T-Cell Ag, 50kD, Clone: MRC OX-40, Mouse Monoclonal antibody-Rat; frozen, IH/flow

  • Alternative name 1
    CD134, OX-40 Ligand, CD4 +'ve activated T-Cell Ag, 50kD, Clone: MRC OX-40, Mab anti-Rat; frozen, IH/flow
  • Alternative name 2
    CD134, OX-40 Ligand, CD4 +'ve activated T-Cell Ag, 50kD, Clone: MRC OX-40, Mab antibody-Rat; frozen, IH/flow
  • Alternative name 3
    CD134, OX-40 Ligand, CD4 +'ve activated T-Cell Ag, 50kD, Clone: MRC OX-40, Mab antibody-Rattus norvegicustus norvegicus; frozen, IH/flow
  • Alternative name 4
    anti-CD134 OX-40 Ligand CD4 +'ve activated T-Cell Ag 50kD Clone: MRC OX-40
  • Alternative name 5
    CD134 OX-40 Ligand CD4 +'ve activated T-Cell Ag 50kD Clone: MRC OX-40
  • Clonality
    Monoclonal Antibody
  • Description
    This is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
  • Storage and handling
    Generally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
  • Advisory
    To keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
  • Properties
    If you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • Additional description
    CD4+ T helper cell marker or cluster differentiator of white blood cells in FACS or flow cytometry. Used offer coupled to magnetic beads. GENTAUR makes custom magnetic bead couplings on 50 and 250 nm beads for certain clones. For cells, cell lines and tissues in culture till half confluency. FAS ligand and other ligands are binding to the receptor for signaling pathways for example in apoptosis or JNK signaling. Receptor agonists are often tested for drug development.
  • Test
    Flow cytometry  uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
  • Latin name
    Mus musculus, Rattus norvegicus
  • French translation
    anticorps
  • Gene target
    CD134   OX-40   Ligand   CD4   activated   50kD   MRC   OX-40   Monoclonal   frozen  
  • Gene symbol
    TNFRSF4, CD200, SNORD115-40, HCRT, IGHV7-40, IGKV2D-40, PPID, IGKV2-40, IGLV1-40, IGHVII-40-1
  • Short name
    CD134, OX-40 Ligand, CD4 +'ve activated T- Ag, 50kD, Clone: MRC OX-40, Mouse Monoclonal antibody- ; frozen, IH/
  • Technique
    Mouse monoclonal, Antibody, frozen, Mouse, Flow, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
  • Host
    mouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
  • Species
    Rat, Mouses, Rats
  • Alternative name
    CD134, OX-40 Ligand, CD4 molecule +'ve activated T-cellular Ag, 50kD, clonality: MRC OX-40, Mouse monoclonal (antibody to-)-Rat; -25°C frozen, IH/flow
  • Alternative technique
    antibodies, murine
  • Alternative to gene target
    CD4 molecule, CD4mut, CD4 and IDBG-15034 and ENSG00000010610 and 920, protein homodimerization activity, Cell surfaces, Cd4 and IDBG-188351 and ENSMUSG00000023274 and 12504, CD4 and IDBG-640247 and ENSBTAG00000003255 and 407098
  • Tissue
    cell, frozen
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    CD200 molecule
  • Synonyms gene
  • Synonyms gene name
    • antigen identified by monoclonal antibody MRC OX-2
    • CD200 antigen
  • Synonyms
  • Locus
  • Discovery year
    2001-06-22
  • Entrez gene record
  • Classification
    • CD molecules
    • Immunoglobulin like domain containing
  • VEGA ID
Gene info
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    immunoglobulin heavy variable 7-40 (pseudogene)
  • Synonyms gene name
    • immunoglobulin heavy variable 7-40
    • immunoglobulin heavy variable 7-40 pseudogene
  • GenBank acession
  • Locus
  • Discovery year
    2000-04-04
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Immunoglobulin heavy locus at 14q32.33
  • VEGA ID
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    peptidylprolyl isomerase D
  • Synonyms gene name
    • peptidylprolyl isomerase D (cyclophilin D)
  • Synonyms
  • Synonyms name
  • Locus
  • Discovery year
    1995-08-23
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • Cyclophilin peptidylprolyl isomerases
    • Tetratricopeptide repeat domain containing
  • VEGA ID
Gene info
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    immunoglobulin heavy variable (II)-40-1 (pseudogene)
  • Synonyms gene name
    • immunoglobulin heavy variable (II)-40-1
    • immunoglobulin heavy variable (II)-40-1 pseudogene
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2000-04-17
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Immunoglobulin heavy locus at 14q32.33
  • VEGA ID
MeSH Data
  • Name
  • Concept
    Scope note: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
  • Tree numbers
    • E01.370.225.500.363.342
    • E01.370.225.500.386.350
    • E05.196.712.516.600.240.350
    • E05.200.500.363.342
    • E05.200.500.386.350
    • E05.242.363.342
    • E05.242.386.350
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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