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Human Enoyl Coenzyme A Hydratase 1 Peroxisomal ECH1 ELISA Kit 96 well plate

Human Enoyl Coenzyme A Hydratase 1 Peroxisomal ECH1 ELISA Kit 96 well plate is available 3 times from Bioma labs

EKU08452 | Human Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1) ELISA Kit, 96 well plate size: 10x96T | 7,333.77 USD

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EKU08452 | Human Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1) ELISA Kit, 96 well plate size: 5x96T | 4,058.89 USD

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EKU08452 | Human Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1) ELISA Kit, 96 well plate size: 96T | 966.08 USD

1. Gene info
Identity 3149
Gene ECH1
Long gene name enoyl-CoA hydratase 1
Synonyms gene name
  • enoyl Coenzyme A hydratase 1, peroxisomal
  • enoyl-CoA hydratase 1, peroxisomal
Synonyms
  • HPXEL
Synonyms name
  • delta3,5-delta2,4-dienoyl-coenzyme A isomerase
  • peroxisomal enoyl-CoA hydratase 1
GenBank acession
  • U16660
Locus 19q13.2
Discovery year 1995-04-26
Entrez gene record 1891
Pubmed identfication
  • 7558027
Havana BLAST/BLAT OTTHUMG00000182609
MeSH Data
Name Enzyme-Linked Immunosorbent Assay
Tree numbers
  • E05.478.566.350.170
  • E05.478.566.380.360
  • E05.478.583.400.170
  • E05.601.470.350.170
  • E05.601.470.380.360
Product images
    #Rat#.png#Human#.jpg
Assay class Sandwich
ELISA's stability The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
ELISA's cross-reactivity This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
Samples to be analyzed Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Estimated production time 7-11 business days
Sensitivity limit 0,058 ng/mL
Alternate gene name HPXEL; Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial
Gene number 1891
Assay duration 3 hours
QC The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Gene name Enoyl Coenzyme A Hydratase 1, Peroxisomal
Protein number Q13011
Detection limits 0.156-10 ng/mL
Protocol Please see ELISA's datasheet, otherwise contact us
Use for 8 months
Notes For research use only. Not for diagnostic procedures.
Storage recommendation -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Research main area Enzyme & Kinase;
Shipping requirements Blue ice
ELISA detection Colorimetric
Verified reactivity Homo sapiens (Human)
ELISA's specificity This assay has high sensitivity and excellent specificity for detection of Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1). No significant cross-reactivity or interference between Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1) and analogues was observed.
Precision of the test Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
Alternate protein number Please refer to SwissProt
Assay principle The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Warnings The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Verified applications ELISA
Properties E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Description A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.
Short name Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1) ELISA Kit, 96
Technique ELISA kit, ELISA
Host antigen assay ELISA kit 1 Kit (96 Wells)
Species Human
Alternative name H. sapiens Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1) Enzyme-linked immunosorbent assay reagent, 96 well plate
Alternative technique enzymes
Alternative to gene target v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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