Anti-CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781), Biotin conjugate

• Description:

  DC-SIGN is a transmembrane receptor that is expressed on the surface of dendritic cells and macrophages. It is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens ranging from parasites to viruses. The protein is organized into three distinct domains: an N-terminal transmembrane domain, a tandem-repeat neck domain and C-type lectin carbohydrate recognition domain. The extracellular region consisting of the C-type lectin and neck domains has a dual function as a pathogen recognition receptor and a cell adhesion receptor by binding carbohydrate ligands on the surface of microbes and endogenous cells. The neck region is important for homo-oligomerization, which allows the receptor to bind multivalent ligands with high avidity.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  CD209; CDSIGN; CIRE; CLEC4L; DC-SIGN; DC-SIGN1; DCSIGN; Dendritic cell-specific ICAM-3 Grabbing Non-integrin 1; HIV GP120 Binding Protein; SIGN-R1; SIGNR5

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  CD209

• Gene ID:

  30835

• NCBI Gene ID:

  278694

• UniProt:

  Q9NNX6

• Cellular Locus:

  Plasma membrane|Secreted (extracellular)

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Recombinant human CD209 protein fragment

• Target Antigen:

  CD209 | DC-SIGN

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  C209/1781

• Conjugation:

  Biotin

• Source:

  Animal

• Applications:

  IHC, FFPE (verified) | WB (verified)

• Validated Applications:

  IHC, FFPE, WB

• Field of Research:

  Immunology

• Positive Control:

  THP-1 or HeLa Cells. Small Intestine, Uterus or Lymph Node.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  45 kDa

• Additionnal Information:

  Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris with 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min|Immunohistology (formalin) 0.5 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Immunofluorescence 1-2 ug/mL|Optimal dilution for a specific application should be determined by user

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4