Anti-Podocalyxin(3D3), CF488A conjugate

• Description:

  Podocalyxin is a member of the CD34 transmembrane sialomucin family. It is highly on kidney podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  PODXL, PDXL, GCTM-2; Gp200; PCLP1; Pcx; Podocalyxin-like protein 1

• CAS Number:

  9007-83-4

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  PODXL

• Gene ID:

  5420

• NCBI Gene ID:

  732423

• UniProt:

  O00592

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human, Rabbit, Rat

• Immunogen:

  A recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of human podocalyxin

• Target Antigen:

  Podocalyxin

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Clone:

  3D3

• Conjugation:

  CF488A

• Source:

  Animal

• Applications:

  Flow, intracellular (verified) | IF (verified) | IHC, FFPE (verified) | WB (verified)

• Validated Applications:

  FC, IF, IHC, FFPE, WB

• Field of Research:

  Developmental biology

• Positive Control:

  HeLa, Raji, Jurkat cells, Angiosarcoma, Breast, Prostate, Liver, Pancreatic, Kidney

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  165-170 kDa

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Immunoprecipitation 1-2 ug/500 ug protein|Optimal dilution for a specific application should be determined by user

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years