Protein A Spin Antibody Purification Kit

• Catalog number
  K942-10
• Price
  Please ask
• Size
  10 columns

• Description
  The kit can be used to purify antibodies in serum, ascites and cell culture medium from various species such as human, mouse, rat, goat and rabbit.
• Summary
  The kit can be used to purify antibodies in serum, ascites and cell culture medium from various species such as human, mouse, rat, goat and rabbit.
• Species Reactivity
  human, mouse, rat, goat and rabbit
• Applications
  Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants.
• Sample Type
  serum, ascites and cell culture medium
• Features Benefits
  Ready-to-use pre-packed spin columns of 0.1 ml bead volume in 20% Ethanol. HIGH BINDING CAPACITY: Binding of IgG ≥ 30 mg human, mouse or rabbit IgG mg/ml Protein A-Agarose
• Storage Conditions
  4°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Protein A beads have been widely used for IgG purification for their ability to bind immunoglobulins. BioVision’s Protein A (Cat. No. 6500) is a genetically engineered protein containing five IgG-binding regions of native Protein A. Maximum IgG specificity is achieved by eliminating its cell wall binding region, albumin binding region, and other non-specific regions. Hi-BindTM Protein A-Agarose beads (Cat. No. 6520) for purification of IgG contain covalently coupled recombinant Protein A to 6% cross-linked Agarose beads, the most popular resin for protein purification. BioVision’s Protein A Mini Antibody Purification Kit is a simple, ready to use kit containing all necessary buffers and pre-packed columns to purify up to 30 mg/ml of IgG. The kit can be used to purify antibodies in serum, ascites and cell culture medium from various species such as human, mouse, rat, goat and rabbit.
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Test
  Antibodies are affinity purified with an antigen coated column or protein A or G agarose or beads. DNA is purified with endotoxin free silica columns or anion exchange resins. Biovision supplies purification kits and ultra pure reagents.
• French translation
  anticorps

• Gene target
  Protein   Spin   Kit  
• Short name
  Protein A Spin Antibody Purification Kit
• Technique
  Purification, Antibody, antibodies against human proteins, antibodies for, purified
• Alternative name
  Protein A Spin (antibody to-) purification reagent
• Alternative technique
  purifications, antibodies, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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