Canine Integrin alpha 11 beta 1 (ITGA11&ITGB1) Heterodimer Protein, His Tag&Tag Free

• Background:

  Integrin alpha 11 beta 1 is a receptor for collagen. The alpha11 cDNA encodes a mature protein with a large 1120-residue extracellular domain that contains an I-domain of 207 residues and is linked by a transmembrane domain to a short cytoplasmic domain of 24 amino acids. The deduced alpha11 protein shows the typical structural features of integrin alpha-subunits and is similar to a distinct group of alpha-subunits from collagen-binding integrins.

• Description:

  Canine ITGA11&ITGB1 Heterodimer Protein, His Tag&Tag Free (IT1-C52W9) is expressed from human 293 cells (HEK293). It contains AA Asp 21 - Pro 1142 (ITGA11) & Asp 23 - Asp 728 (ITGB1) (Accession # A0A8I3S131-1 (ITGA11) & A0A8C0PYR6-1 (ITGB1)).

• Specifications:

  Canine ITGA1&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA1 and ITGB1, has a calculated MW of 132.6 kDa (ITGA11) and 83.2 kDa (ITGB1). Subunit ITGA1 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus.

• Expression Region:

  Asp 21 - Pro 1142 (ITGA11) & Asp 23 - Asp 728 (ITGB1)

• Host:

  HEK 293

• Target:

  Integrin alpha 11 beta 1

• Conjugation:

  Unconjugated

• Tag:

  C-10×His | Native

• Source:

  Canine

• Stability:

  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  90%

• Bioactivity:

  Immobilized Native Human Collagen I protein at 10 μg/mL (100 μL/well) can bind Canine ITGA11&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-C52W9) with a linear range of 0.078-5 μg/mL (QC tested).

• Format:

  Powder

• Buffer:

  50 mM Tris,150 mM NaCl, pH7.5

• Additives:

  Trehalose

• Molecular Weight:

  132.6 kDa (ITGA1) & 83.2 kDa (ITGB1)

• Shipping Conditions:

  Room Temperature

• Storage Conditions:

  -20°C